Detailed information of TA system TA166311

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pasABC/RelE-HTH
• Location: 1688..2162
• Replicon: plasmid pKPCZA02_3
• Accession: NZ_CP058229
• Organism: Klebsiella pneumoniae strain KPCZA02

Toxin (Protein)

• Gene name: pasB
• Uniprot ID: A0A8J3DT22
• Locus tag: HWQ20_RS27620
• Protein ID: WP_004197762.1
• Coordinates: 1896..2162 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: pasA
• Uniprot ID: S1EJF5
• Locus tag: HWQ20_RS27615
• Protein ID: WP_000879771.1
• Coordinates: 1688..1912 (+)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HWQ20_RS27605	293..802	+	510	WP_032433972.1	MbeB family mobilization protein	-
HWQ20_RS27610 (HWQ20_27610)	809..1021	+	213	WP_021527181.1	MbeD family mobilization/exclusion protein	-
HWQ20_RS28550	1397..1525	-	129	WP_000523570.1	hypothetical protein	-
HWQ20_RS27615 (HWQ20_27615)	1688..1912	+	225	WP_000879771.1	DUF6290 family protein	Antitoxin
HWQ20_RS27620 (HWQ20_27620)	1896..2162	+	267	WP_004197762.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HWQ20_RS27625 (HWQ20_27625)	2232..2582	-	351	WP_004197756.1	hypothetical protein	-
HWQ20_RS27630 (HWQ20_27630)	2648..3193	-	546	WP_023317533.1	hypothetical protein	-
HWQ20_RS27635 (HWQ20_27635)	3219..3545	-	327	WP_050516389.1	hypothetical protein	-
HWQ20_RS27640 (HWQ20_27640)	4701..4877	+	177	WP_004197757.1	hypothetical protein	-
HWQ20_RS27645 (HWQ20_27645)	4867..5211	+	345	WP_062955149.1	MobC family plasmid mobilization relaxosome protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..5595	5595

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-87)

Antitoxin

• Relaxosome protein TraY (4-45)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10342.96 Da
• Isoelectric Point: 9.8727

>T166311 WP_004197762.1 NZ_CP058229:1896-2162 [Klebsiella pneumoniae]
MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGKPLKGELGEFWRYRVGDYRVLCEIRDDELVILAATI
GHRREVYD

Nucleotide

• Length: 267 bp

>T166311 NZ_CP074120:1779262-1779480 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8369.28 Da
• Isoelectric Point: 4.6533

>AT166311 WP_000879771.1 NZ_CP058229:1688-1912 [Klebsiella pneumoniae]
MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYLSAETAARVRRGDEAVHSSEDVRKSLGLDD

Nucleotide

• Length: 225 bp

>AT166311 NZ_CP074120:1778862-1779236 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Antitoxin

• AlphaFold DB: S1EJF5