Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 7001..7644 Replicon plasmid pKPCZA02_2
Accession NZ_CP058228
Organism Klebsiella pneumoniae strain KPCZA02

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag HWQ20_RS26640 Protein ID WP_001044770.1
Coordinates 7228..7644 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag HWQ20_RS26635 Protein ID WP_001261282.1
Coordinates 7001..7231 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HWQ20_RS28385 (2190) 2190..2492 + 303 WP_004197636.1 hypothetical protein -
HWQ20_RS26600 (2964) 2964..3758 - 795 WP_004197635.1 site-specific integrase -
HWQ20_RS26605 (3956) 3956..4972 - 1017 WP_017899884.1 hypothetical protein -
HWQ20_RS26610 (4983) 4983..5297 - 315 WP_053389906.1 hypothetical protein -
HWQ20_RS26615 (5324) 5324..5719 - 396 WP_017899885.1 hypothetical protein -
HWQ20_RS26620 (5888) 5888..6193 - 306 WP_013023785.1 type II toxin-antitoxin system toxin CcdB -
HWQ20_RS26625 (6195) 6195..6413 - 219 WP_001568025.1 type II toxin-antitoxin system antitoxin CcdA -
HWQ20_RS26630 (6583) 6583..7044 - 462 WP_215824896.1 hypothetical protein -
HWQ20_RS26635 (7001) 7001..7231 + 231 WP_001261282.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
HWQ20_RS26640 (7228) 7228..7644 + 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
HWQ20_RS26645 (7718) 7718..9280 + 1563 WP_004206609.1 AAA family ATPase -
HWQ20_RS26650 (9265) 9265..10287 + 1023 WP_000361404.1 helicase UvrD -
HWQ20_RS26655 (10543) 10543..11240 + 698 WP_223174630.1 IS1 family transposase -
HWQ20_RS26660 (11269) 11269..11541 + 273 Protein_15 transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid blaKPC-2 / blaSHV-12 / blaCTX-M-65 / fosA3 / blaTEM-1B / rmtB - 1..149200 149200


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T166304 WP_001044770.1 NZ_CP058228:7228-7644 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T166304 NZ_CP074120:104477-104731 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT166304 WP_001261282.1 NZ_CP058228:7001-7231 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT166304 NZ_CP074120:104229-104480 [Escherichia coli]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References