Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 5888..6413 Replicon plasmid pKPCZA02_2
Accession NZ_CP058228
Organism Klebsiella pneumoniae strain KPCZA02

Toxin (Protein)


Gene name ccdB Uniprot ID D4HQE7
Locus tag HWQ20_RS26620 Protein ID WP_013023785.1
Coordinates 5888..6193 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID W8V2V6
Locus tag HWQ20_RS26625 Protein ID WP_001568025.1
Coordinates 6195..6413 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HWQ20_RS26590 (1567) 1567..2193 + 627 WP_004197649.1 ParA family plasmid-partitioning AAA ATPase -
HWQ20_RS28385 (2190) 2190..2492 + 303 WP_004197636.1 hypothetical protein -
HWQ20_RS26600 (2964) 2964..3758 - 795 WP_004197635.1 site-specific integrase -
HWQ20_RS26605 (3956) 3956..4972 - 1017 WP_017899884.1 hypothetical protein -
HWQ20_RS26610 (4983) 4983..5297 - 315 WP_053389906.1 hypothetical protein -
HWQ20_RS26615 (5324) 5324..5719 - 396 WP_017899885.1 hypothetical protein -
HWQ20_RS26620 (5888) 5888..6193 - 306 WP_013023785.1 type II toxin-antitoxin system toxin CcdB Toxin
HWQ20_RS26625 (6195) 6195..6413 - 219 WP_001568025.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
HWQ20_RS26630 (6583) 6583..7044 - 462 WP_215824896.1 hypothetical protein -
HWQ20_RS26635 (7001) 7001..7231 + 231 WP_001261282.1 type II toxin-antitoxin system VapB family antitoxin -
HWQ20_RS26640 (7228) 7228..7644 + 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin -
HWQ20_RS26645 (7718) 7718..9280 + 1563 WP_004206609.1 AAA family ATPase -
HWQ20_RS26650 (9265) 9265..10287 + 1023 WP_000361404.1 helicase UvrD -
HWQ20_RS26655 (10543) 10543..11240 + 698 WP_223174630.1 IS1 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid blaKPC-2 / blaSHV-12 / blaCTX-M-65 / fosA3 / blaTEM-1B / rmtB - 1..149200 149200


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11615.30 Da        Isoelectric Point: 6.4672

>T166303 WP_013023785.1 NZ_CP058228:c6193-5888 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T166303 NZ_CP074120:104229-104480 [Escherichia coli]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8392.34 Da        Isoelectric Point: 4.7405

>AT166303 WP_001568025.1 NZ_CP058228:c6413-6195 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRSERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT166303 NZ_CP074120:104477-104731 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J4ZZW6

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4ZZP8

References