Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4106601..4107220 Replicon chromosome
Accession NZ_CP058226
Organism Klebsiella pneumoniae strain KPCZA02

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag HWQ20_RS20420 Protein ID WP_002892050.1
Coordinates 4107002..4107220 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag HWQ20_RS20415 Protein ID WP_002892066.1
Coordinates 4106601..4106975 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HWQ20_RS20405 (HWQ20_20405) 4101753..4102946 + 1194 WP_002892072.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
HWQ20_RS20410 (HWQ20_20410) 4102969..4106115 + 3147 WP_020326861.1 multidrug efflux RND transporter permease subunit AcrB -
HWQ20_RS20415 (HWQ20_20415) 4106601..4106975 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
HWQ20_RS20420 (HWQ20_20420) 4107002..4107220 + 219 WP_002892050.1 HHA domain-containing protein Toxin
HWQ20_RS20425 (HWQ20_20425) 4107379..4107945 + 567 WP_002892030.1 maltose O-acetyltransferase -
HWQ20_RS20430 (HWQ20_20430) 4107917..4108057 - 141 WP_004147370.1 hypothetical protein -
HWQ20_RS20435 (HWQ20_20435) 4108078..4108548 + 471 WP_002892026.1 YlaC family protein -
HWQ20_RS20440 (HWQ20_20440) 4108523..4109974 - 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
HWQ20_RS20445 (HWQ20_20445) 4110075..4110773 + 699 WP_002892021.1 GNAT family protein -
HWQ20_RS20450 (HWQ20_20450) 4110770..4110910 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
HWQ20_RS20455 (HWQ20_20455) 4110910..4111173 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T166298 WP_002892050.1 NZ_CP058226:4107002-4107220 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T166298 NZ_CP074116:5054679-5054966 [Klebsiella pneumoniae subsp. pneumoniae]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT166298 WP_002892066.1 NZ_CP058226:4106601-4106975 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT166298 NZ_CP074116:5054440-5054679 [Klebsiella pneumoniae subsp. pneumoniae]
ATGGGTAGCATTAACCTACGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGCGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGACTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References