Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 120479..122047 Replicon plasmid p550_IncA_VIM_1
Accession NZ_CP058224
Organism Escherichia coli strain 550

Toxin (Protein)


Gene name hipA Uniprot ID A0A859R327
Locus tag HWQ66_RS24240 Protein ID WP_024134513.1
Coordinates 120479..121786 (-) Length 436 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A859R2S1
Locus tag HWQ66_RS24245 Protein ID WP_039272366.1
Coordinates 121796..122047 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HWQ66_RS24220 116362..116664 + 303 WP_031611417.1 hypothetical protein -
HWQ66_RS24225 117036..118676 - 1641 WP_031611419.1 PAAR domain-containing protein -
HWQ66_RS24230 118875..119813 + 939 WP_044596315.1 hypothetical protein -
HWQ66_RS24235 119988..120482 - 495 WP_039272364.1 hypothetical protein -
HWQ66_RS24240 120479..121786 - 1308 WP_024134513.1 type II toxin-antitoxin system HipA family toxin Toxin
HWQ66_RS24245 121796..122047 - 252 WP_039272366.1 helix-turn-helix domain-containing protein Antitoxin
HWQ66_RS24250 122412..123416 - 1005 WP_039272368.1 tyrosine-type recombinase/integrase -
HWQ66_RS24255 123419..123982 - 564 WP_001132404.1 hypothetical protein -
HWQ66_RS24260 124001..124282 - 282 WP_000575656.1 hypothetical protein -
HWQ66_RS24265 124543..125145 + 603 WP_039272372.1 hypothetical protein -
HWQ66_RS24270 125161..125613 - 453 WP_001553092.1 hypothetical protein -
HWQ66_RS24275 125669..126394 - 726 WP_001553093.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid sul2 / aph(3'')-Ib / aph(6)-Id / qnrS1 / sul1 / qacE / catB2 / ant(3'')-Ia / aph(3')-XV / blaVIM-1 / blaSHV-12 / mph(A) / dfrA14 - 1..162608 162608


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(4-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(14-64)


Sequences

Toxin        


Download         Length: 436 a.a.        Molecular weight: 47950.12 Da        Isoelectric Point: 6.2444

>T166283 WP_024134513.1 NZ_CP058224:c121786-120479 [Escherichia coli]
MQTLTVAMNGEVVGTLYRDNKGAMSFQYAPEWISEPGSRAISMSLPLGHGRITGSEVFNFFSNLLPDSEAIIARMQARFR
VETTHPFDLLASVGRDCVGAIQLYSPDADIPTVMETLAEPLDEAQIEQLLEGYQEAPLGMAEDADFRISIAGAQEKTALL
WYQDRWQRPLGSTPTSHIFKLPIGKIVQNNIDLSESCENEWLCLRIAKAFGFDVANADLATFGKKKVLVVQRFDRRWSKS
GEWLLRLPQEDFCQALGIAPALKYESHGGPGIADAMKLLLGSRLAAKDREQFFKSQILFWMLAAIDGHGKNFSIFIEPAS
SFRMTPLYDVMSAFPIFKSGGIAAQKAKMGMALQGKNRQYHFAMIQPRHFISTAAHVGFPQDTAAQLMFDMATKTEEVVA
TVAAELPAGFPEHISSAIFEGLSSQAAKILKGVAK

Download         Length: 1308 bp

>T166283 NZ_CP074112:1704313-1704672 [Idiomarina loihiensis]
ATGTGGACAATACGAACTACGGATAGGTTTGATGCTTGGTTTGATGCATTAGATGATACAGACAAAGAAAACGTTATCGC
TTCGTTGCTTCTATTGCAAAGTGAAGGACCTTTTCTGTCGAGACCTTATGCAGATACTTTATATGGTTCTGTTCACAGCA
ATCTTAAAGAATTACGAATTCAATCAAAAGGTAAGCCCATCAGGGTCTGTTTTGCATTTGATCTAAGCCGAACGGCGATT
ATCTTGTGTGCAGCGCATAAAAACCGCAACACTCGGCTTTTTTATCAAACGATGATCGCGACAGCAGACCAAGAGTTTAC
TCGACATTTAATCGCTATGGGGCAGAAAGACAATGGCTAG

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9369.79 Da        Isoelectric Point: 9.8930

>AT166283 WP_039272366.1 NZ_CP058224:c122047-121796 [Escherichia coli]
MKVTSPSLLAAAVRDQRKLSKLTQSEAAKQVGIKQTTVSDFELRPESTKLETLFKLLSALDLELHVVKRGSTLDDSKVWD
REW

Download         Length: 252 bp

>AT166283 NZ_CP074112:1704665-1704967 [Idiomarina loihiensis]
ATGGCTAGAACACTCGAGCAATTAATAGCCAACGAAAAACCAGAGCTCATTGCAAGAGCCAGCAAGAAGGCTGATGCTAT
GCTGCTAACTATTCGGCTAAGTGAGATCAGGAAGCTGGTTAATAAAACCCAAGTTGATCTAGCAAACTCTATGGGAATTA
AACAACCCACGGTAGCAGGTATGGAAAAAACTGGAGCCGATATTAAGTTATCTAGCTTAAAAAAATATATCGAAGCTTGT
GGTGCTCATTTAAAAGTGGATATTGAACTTCCTGACGGGAGCCATCACCAATTTAGCTTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A859R327

Antitoxin

Source ID Structure
AlphaFold DB A0A859R2S1

References