Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4838348..4838966 Replicon chromosome
Accession NZ_CP058223
Organism Escherichia coli strain 550

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag HWQ66_RS22960 Protein ID WP_001291435.1
Coordinates 4838748..4838966 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag HWQ66_RS22955 Protein ID WP_000344800.1
Coordinates 4838348..4838722 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HWQ66_RS22945 4833437..4834630 + 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
HWQ66_RS22950 4834653..4837802 + 3150 WP_001132480.1 multidrug efflux RND transporter permease subunit -
HWQ66_RS22955 4838348..4838722 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
HWQ66_RS22960 4838748..4838966 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
HWQ66_RS22965 4839138..4839689 + 552 WP_000102559.1 maltose O-acetyltransferase -
HWQ66_RS22970 4839805..4840275 + 471 WP_000136192.1 YlaC family protein -
HWQ66_RS22975 4840439..4841989 + 1551 WP_001389864.1 cyclic-guanylate-specific phosphodiesterase PdeB -
HWQ66_RS22980 4842031..4842384 - 354 WP_000878140.1 DUF1428 domain-containing protein -
HWQ66_RS22990 4842763..4843074 + 312 WP_000409911.1 MGMT family protein -
HWQ66_RS22995 4843105..4843677 - 573 WP_000779826.1 lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T166282 WP_001291435.1 NZ_CP058223:4838748-4838966 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T166282 NZ_CP074112:c1179798-1179553 [Idiomarina loihiensis]
ATTCGGGATCGTGAGCAAATTTATGACTTTATCGAAAATGATAACCCAGTAGCGGCGCTTCAACTAGATAATCAAGTCTC
GTCAAGCGTAGCAAATCTGTCTGAATATCCAGAAATAGGCCGTGAAGGACGGGTTCTTGGAACTCGAGAGCTTGTAGTGC
ATGCAAACTATATTGTCGTGTACGATTTCAGTAAGGACGCCCTTAGAATTTTACGAATCCTACATGCGGCTATGAAGTGG
CCTTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT166282 WP_000344800.1 NZ_CP058223:4838348-4838722 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT166282 NZ_CP074112:c1180109-1179822 [Idiomarina loihiensis]
ATGAGCGATGCAACATTTACGTTCCGCGTTAACGATGAGTTGAAAAGTGAATTTTCAAAAGCCGCACGCTCGCGCGATCG
CAGTAGTGCGCAATTGCTACGTGACTTTATGCGTGAGTACGTTCAGCAACAAGAGCGGTTGGCTGAGCACGATAGCTGGT
TTAGGGAGCAAGTGAAGCAAGGGCTTGATTCTGCTAACGCTGGGAATTTGGTTCCAGCGGGTGACGTTGAGGCTAAGTTT
GCGGCACGCAGAGCGTTAACGCAGCGTAACATCGATAAGCAACGATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References