Detailed information of TA system TA166281

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 4377199..4377904
• Replicon: chromosome
• Accession: NZ_CP058223
• Organism: Escherichia coli strain 550

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1F406
• Locus tag: HWQ66_RS20780
• Protein ID: WP_000539521.1
• Coordinates: 4377199..4377585 (+)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: HWQ66_RS20785
• Protein ID: WP_001280945.1
• Coordinates: 4377575..4377904 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HWQ66_RS20760	4373203..4373829	+	627	WP_001295292.1	glutathione S-transferase GstB	-
HWQ66_RS20765	4373826..4374941	-	1116	WP_000555038.1	aldose sugar dehydrogenase YliI	-
HWQ66_RS20770	4375052..4375435	-	384	WP_000497137.1	biofilm formation regulator BssR	-
HWQ66_RS20775	4375648..4376973	+	1326	WP_000049367.1	30S ribosomal protein S12 methylthiotransferase RimO	-
HWQ66_RS20780	4377199..4377585	+	387	WP_000539521.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HWQ66_RS20785	4377575..4377904	+	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
HWQ66_RS20790	4377974..4379302	-	1329	WP_000086879.1	GGDEF domain-containing protein	-
HWQ66_RS20795	4379310..4381658	-	2349	WP_001322378.1	EAL domain-containing protein	-
HWQ66_RS20800	4381836..4382747	-	912	WP_001236042.1	glutathione ABC transporter permease GsiD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14293.45 Da
• Isoelectric Point: 9.9296

>T166281 WP_000539521.1 NZ_CP058223:4377199-4377585 [Escherichia coli]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T166281 NZ_CP074109:414466-414807 [Enterococcus faecium]
GTGTCAGAAGAAAGAATTTATATTCCTAAAAAAGGTGATATTGTATGGATTGATTTTGATCCCTCAGTAGGAAAAGAAAT
TCAAAAAAGACGACCAGGACTAGTCGTCTCTCGTTATGAATTTAACCGTAAAACAATGTTTTCGGTCATATGTCCAATTA
CTTCCACTATAAAGAATTTACCGACACGTTACTCATTACCTGAAGAGCTAGATATAAAAGGACAAGTAATTATTACACAA
TTAAAATCTCTTGATTTTAAGGAACGAAAACTCAAAAAAATAGAAAACTTACCATTGCAAGACATGGCTAAAATTGATCA
GATTATACAGTATATGTTTTAA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT166281 WP_001280945.1 NZ_CP058223:4377575-4377904 [Escherichia coli]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT166281 NZ_CP074109:414236-414466 [Enterococcus faecium]
ATGCTTGTTACAAAATCTAGATTACAAGGAAGTTCTGTAGTGGTAACTTTACCTTCTGATAACGGAAAGAAACCTTCAGA
AAACCAAGAATATATCGTTGTGTATTCTGATGATGGAACAATTACGTTAGTTCCAAAAATTGAAGATCCATTTAGTGGTG
GGGAAGAGGCCGAGTATTATGAGAAAGACGAATGGGAAGATTTAACTCCTGAAGGGAGAGAAATTTTATAG

Structures

Toxin

• AlphaFold DB: A0A829GCT6