Detailed information of TA system TA166280

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4224074..4224908
• Replicon: chromosome
• Accession: NZ_CP058223
• Organism: Escherichia coli strain 550

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: HWQ66_RS20055
• Protein ID: WP_115725052.1
• Coordinates: 4224074..4224451 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: HWQ66_RS20060
• Protein ID: WP_175064154.1
• Coordinates: 4224540..4224908 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HWQ66_RS20025	4220754..4221458	+	705	WP_001241678.1	leucyl/phenylalanyl-tRNA--protein transferase	-
HWQ66_RS20030	4221743..4221961	+	219	WP_001040187.1	translation initiation factor IF-1	-
HWQ66_RS20040	4222452..4223294	-	843	WP_115725053.1	DUF4942 domain-containing protein	-
HWQ66_RS20045	4223380..4223577	-	198	WP_000839250.1	DUF957 domain-containing protein	-
HWQ66_RS20050	4223589..4224077	-	489	WP_096847166.1	hypothetical protein	-
HWQ66_RS20055	4224074..4224451	-	378	WP_115725052.1	TA system toxin CbtA family protein	Toxin
HWQ66_RS20060	4224540..4224908	-	369	WP_175064154.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HWQ66_RS20065	4224958..4225602	-	645	WP_115725050.1	antitoxin of toxin-antitoxin stability system	-
HWQ66_RS20070	4225621..4225842	-	222	WP_000692312.1	DUF987 domain-containing protein	-
HWQ66_RS20075	4225905..4226381	-	477	WP_001186193.1	RadC family protein	-
HWQ66_RS20080	4226397..4226870	-	474	WP_001313575.1	antirestriction protein	-
HWQ66_RS20085	4227188..4228006	-	819	WP_175064161.1	DUF945 domain-containing protein	-
HWQ66_RS20090	4228285..4228740	-	456	WP_175064160.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (19-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14180.10 Da
• Isoelectric Point: 6.8528

>T166280 WP_115725052.1 NZ_CP058223:c4224451-4224074 [Escherichia coli]
MKTLPDTHVREASRCPSPVTIWQTLLSRLLDQHYGLTLDDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPEF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNGITQGKHPEAKQ

Nucleotide

• Length: 378 bp

>T166280 NZ_CP058223:c4224451-4224074 [Escherichia coli]
ATGAAAACATTACCCGACACACACGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGTTGTC
CCGGTTACTGGACCAGCATTACGGCCTTACGCTGGATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGAATTC
AGTGCCTGTACCCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCCCGCCGGGCGACAGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATGGCATTACCCAGGGGAAGCATCCGGAGGCGAAACAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13976.90 Da
• Isoelectric Point: 7.3213

>AT166280 WP_175064154.1 NZ_CP058223:c4224908-4224540 [Escherichia coli]
VSDKLHETNYPDDHNDRIWWGLPCTVTPCFGARLVQEGNRLHYLANRAGIRGRFRDADAYHLDQAFPLLMKQLELMLTSG
ELNPRYQHTVTLYARGLTCKADTLSSCGYVYLAVYPTPEMKN

Nucleotide

• Length: 369 bp

>AT166280 NZ_CP058223:c4224908-4224540 [Escherichia coli]
GTGTCAGACAAGCTCCATGAGACAAATTATCCCGACGATCATAACGACCGCATCTGGTGGGGACTCCCCTGCACGGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCAACCGTGCCGGTATCAGAGGCCGGT
TCAGGGACGCAGATGCATACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCTATCAGCATACCGTCACGCTGTATGCCAGAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA