Detailed information of TA system TA166279

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 4030579..4031363
• Replicon: chromosome
• Accession: NZ_CP058223
• Organism: Escherichia coli strain 550

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: V0T0H9
• Locus tag: HWQ66_RS19140
• Protein ID: WP_000613626.1
• Coordinates: 4030869..4031363 (+)
• Length: 165 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: L4JCW6
• Locus tag: HWQ66_RS19135
• Protein ID: WP_001110447.1
• Coordinates: 4030579..4030872 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HWQ66_RS19125	4025729..4026688	-	960	WP_000846342.1	23S rRNA pseudouridine(955/2504/2580) synthase RluC	-
HWQ66_RS19130	4027261..4030446	+	3186	WP_000827427.1	ribonuclease E	-
HWQ66_RS19135	4030579..4030872	+	294	WP_001110447.1	DUF1778 domain-containing protein	Antitoxin
HWQ66_RS19140	4030869..4031363	+	495	WP_000613626.1	GNAT family N-acetyltransferase	Toxin
HWQ66_RS19145	4031458..4032411	-	954	WP_001212768.1	flagellar hook-associated protein FlgL	-
HWQ66_RS19150	4032423..4034066	-	1644	WP_000096524.1	flagellar hook-associated protein FlgK	-
HWQ66_RS19155	4034132..4035073	-	942	WP_001309406.1	flagellar assembly peptidoglycan hydrolase FlgJ	-
HWQ66_RS19160	4035073..4036170	-	1098	WP_000589320.1	flagellar basal body P-ring protein FlgI	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (81-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-88)

Sequences

Toxin

Protein

• Length: 165 a.a.
• Molecular weight: 18084.00 Da
• Isoelectric Point: 8.2617

>T166279 WP_000613626.1 NZ_CP058223:4030869-4031363 [Escherichia coli]
MIQAPEPLMARHQFTSFCSGVETMDNWLKQRALKNQLAGASRTFVSCDTYSNVLAYYSLASSAVETYVATGRFRRNMPEP
IPVVVLGRLAIDKSLQGQGIGRAMVRDAGLRVLQAAEVIGIRGMLVHALSDQAREFYLRVGFEPSPVDSMILMATLADLQ
ECLK

Nucleotide

• Length: 495 bp

>T166279 NZ_CP074107:414904-415245 [Enterococcus faecium]
GTGTCAGAAGAAAGAATTTATATTCCTAAAAAAGGTGATATTGTATGGATTGATTTTGATCCCTCAGTAGGAAAAGAAAT
TCAAAAAAGACGACCAGGACTAGTCGTCTCTCGTTATGAATTTAACCGTAAAACAATGTTTTCGGTCATATGTCCAATTA
CTTCCACTATAAAGAATTTACCGACACGTTACTCATTACCTGAAGAGCTAGATATAAAAGGACAAGTAATTATTACACAA
TTAAAATCTCTTGATTTTAAGGAACGAAAACTCAAAAAAATAGAAAACTTACCATTGCAAGACATGGCTAAAATTGATCA
GATTATACAGTATATGTTTTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11109.69 Da
• Isoelectric Point: 10.5423

>AT166279 WP_001110447.1 NZ_CP058223:4030579-4030872 [Escherichia coli]
MPAANNIPTRRESLNLRIKPAERDLIDRAAKARGKNRTEFVLEAARAAAEEALIEQRIFRIDSDDYQLFLNRLDQPPAPN
AALRKTMQTPAPWEQKK

Nucleotide

• Length: 294 bp

>AT166279 NZ_CP074107:414674-414904 [Enterococcus faecium]
ATGCTTGTTACAAAATCTAGATTACAAGGAAGTTCTGTAGTGGTAACTTTACCTTCTGATAACGGAAAGAAACCTTCAGA
AAACCAAGAATATATCGTTGTGTATTCTGATGATGGAACAATTACGTTAGTTCCAAAAATTGAAGATCCATTTAGTGGTG
GGGAAGAGGCCGAGTATTATGAGAAAGACGAATGGGAAGATTTAACTCCTGAAGGGAGAGAAATTTTATAG

Structures

Toxin

• AlphaFold DB: V0T0H9

Antitoxin

• AlphaFold DB: L4JCW6