Detailed information of TA system TA166278

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 3685246..3685884
• Replicon: chromosome
• Accession: NZ_CP058223
• Organism: Escherichia coli strain 550

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: B7N4J4
• Locus tag: HWQ66_RS17350
• Protein ID: WP_000813797.1
• Coordinates: 3685708..3685884 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: HWQ66_RS17345
• Protein ID: WP_076611057.1
• Coordinates: 3685246..3685662 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HWQ66_RS17325	3680398..3681339	-	942	WP_001251335.1	ABC transporter permease	-
HWQ66_RS17330	3681340..3682353	-	1014	WP_000220413.1	ABC transporter ATP-binding protein	-
HWQ66_RS17335	3682371..3683516	-	1146	WP_000047447.1	ABC transporter substrate-binding protein	-
HWQ66_RS17340	3683761..3685167	-	1407	WP_000760613.1	PLP-dependent aminotransferase family protein	-
HWQ66_RS17345	3685246..3685662	-	417	WP_076611057.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
HWQ66_RS17350	3685708..3685884	-	177	WP_000813797.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
HWQ66_RS17355	3686106..3686336	+	231	WP_000494244.1	YncJ family protein	-
HWQ66_RS17360	3686428..3688389	-	1962	WP_001309488.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
HWQ66_RS17365	3688462..3688998	-	537	WP_000429161.1	DNA-binding transcriptional regulator SutR	-
HWQ66_RS17370	3689051..3690262	+	1212	WP_001323162.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6724.76 Da
• Isoelectric Point: 11.5666

>T166278 WP_000813797.1 NZ_CP058223:c3685884-3685708 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFQGRRSVMPRHPSDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T166278 NZ_CP074107:173814-174080 [Enterococcus faecium]
ATGAGTAACTATACAGTCGCAATCAAAAACTCTGCGAAAGTGGATTTAAGAAAAATCAAGCAATCGAATTTAAAGAAACA
ATTTGAAGAAGTGATTCAGACATTGAAGGAAGATCCATATATGCCCACGCAGTCTTTTGAAAAGCTGAGACCTACACATG
AAGGCAGATACTCACGCAGGCTGAATAGACAGCATCGAGTGGTTTACAAAGTTGATGAGGAAAATAAAGTTGTGGAAATT
TATTCTGCCTGGACACATTATGAGTGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15251.65 Da
• Isoelectric Point: 4.5908

>AT166278 WP_076611057.1 NZ_CP058223:c3685662-3685246 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLTNNDHFIEVPLSIASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT166278 NZ_CP074107:173551-173814 [Enterococcus faecium]
ATGGCTAATACAACGATGAATCCTAGCACAGCTCGTAAAAATTTTTATCAGTTATTGAAAGAGGTAAATGAAAATCATAC
TGAAATCGAGATTATTAGTGATCGTAGTGGAAACAATGCTGTATTGATTGGGCTAGAAGACTGGAGAGCGATTCAAGAAA
CCCTTTTTCTTGAGCAAACAGGTACTTTAGATAAAGTACGAGACCGCGAAAAAGACGACAGTGGCTTTACGAATATCGAT
GATATTGATTGGGAAGCTCTTTGA

Structures

Toxin

• AlphaFold DB: A0A829J715