Detailed information of TA system TA166265

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 2013613..2014445
• Replicon: chromosome
• Accession: NZ_CP058223
• Organism: Escherichia coli strain 550

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A3P5UBZ5
• Locus tag: HWQ66_RS09465
• Protein ID: WP_001094454.1
• Coordinates: 2013613..2013987 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A3G8RJV8
• Locus tag: HWQ66_RS09470
• Protein ID: WP_001320394.1
• Coordinates: 2014077..2014445 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HWQ66_RS09435	2008726..2009874	-	1149	WP_000905922.1	capsule polysaccharide export inner-membrane protein KpsE	-
HWQ66_RS09440	2009946..2010929	-	984	WP_001298261.1	KpsF/GutQ family sugar-phosphate isomerase	-
HWQ66_RS09445	2011740..2011910	-	171	Protein_1833	IS110 family transposase	-
HWQ66_RS09450	2012253..2012822	-	570	WP_001290250.1	DUF4942 domain-containing protein	-
HWQ66_RS09455	2012919..2013116	-	198	WP_000839276.1	DUF957 domain-containing protein	-
HWQ66_RS09460	2013128..2013616	-	489	WP_000777545.1	hypothetical protein	-
HWQ66_RS09465	2013613..2013987	-	375	WP_001094454.1	TA system toxin CbtA family protein	Toxin
HWQ66_RS09470	2014077..2014445	-	369	WP_001320394.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HWQ66_RS09475	2014495..2015138	-	644	Protein_1839	antitoxin of toxin-antitoxin stability system	-
HWQ66_RS09480	2015157..2015378	-	222	WP_000692300.1	DUF987 domain-containing protein	-
HWQ66_RS09485	2015441..2015917	-	477	WP_001347688.1	RadC family protein	-
HWQ66_RS09490	2015933..2016418	-	486	WP_029701480.1	antirestriction protein	-
HWQ66_RS09495	2016473..2017291	-	819	WP_001234642.1	DUF945 domain-containing protein	-
HWQ66_RS09500	2017391..2017624	-	234	WP_001119719.1	DUF905 family protein	-
HWQ66_RS09505	2017703..2018158	-	456	WP_000581493.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 14059.10 Da
• Isoelectric Point: 9.2447

>T166265 WP_001094454.1 NZ_CP058223:c2013987-2013613 [Escherichia coli]
MNTLPDTHVRKASRCPSPVTIWQTLLSRLLGQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEKR

Nucleotide

• Length: 375 bp

>T166265 NZ_CP074098:c3680388-3680113 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGGGGCAAAGGGAAATTGAATATTCGGGACAGTTCCAGAAAGATGTAAAACGGGCACAAAAGCGTCATAAGGATGTCGG
CAAACTTAAAACACTTATGACGCTTCTGATTCACCACCCTTTTCCCCTTCCTGCCATTTATAAAGATCACCCGTTACAAG
GTTCATATAGCGGATACCGGGATGCCCATATTGAGCCTGACTGGATTCTTATTTATAAGATAACGGATGAATGCCTGCGA
TTTGAACGAACAGGAACGCATGCCGATTTATTTTAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13623.22 Da
• Isoelectric Point: 6.4783

>AT166265 WP_001320394.1 NZ_CP058223:c2014445-2014077 [Escherichia coli]
VSDTFSGTTHPDDNHDRPWWGLPSTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADANHLDQAFPLLMKQLELMFTSS
ELNPHRQNTVTLYAKGLTCHADTLGSCGYVYLAVYPTPETKQ

Nucleotide

• Length: 369 bp

>AT166265 NZ_CP074098:c3680651-3680391 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGGCTGCAAATGCGCTTGTTCGTGCCCGTATCGACGAAACGCTGAAGGACCAGGCGGCAGATGTCCTGGCTGAAATGGG
GTTAACCATTTCTGATTTGATACGGATTACACTGACTAAAGTTGCGCGTGAAAAGGCATTGCCTTTTGATTTGCGCATAC
CCAATGAGTTAACGTCCCGAACGATAGAAAATAGCGAAGCTGGCGTTGATATTCATAAAGCCAAAGATGCTGATGATTTG
TTTGACCAGTTAGGGATCTGA

Structures

Toxin

• AlphaFold DB: A0A3P5UBZ5

Antitoxin

• AlphaFold DB: A0A3G8RJV8