Detailed information of TA system TA166261

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 1327203..1327815
• Replicon: chromosome
• Accession: NZ_CP058223
• Organism: Escherichia coli strain 550

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: U9YXE2
• Locus tag: HWQ66_RS06105
• Protein ID: WP_000833473.1
• Coordinates: 1327203..1327388 (+)
• Length: 62 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: L4IWR9
• Locus tag: HWQ66_RS06110
• Protein ID: WP_000499744.1
• Coordinates: 1327405..1327815 (+)
• Length: 137 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HWQ66_RS06095	1324035..1325186	+	1152	WP_000741501.1	L-threonine dehydrogenase	-
HWQ66_RS06100	1325387..1326925	+	1539	WP_001309845.1	aldehyde dehydrogenase AldB	-
HWQ66_RS06105	1327203..1327388	+	186	WP_000833473.1	type II toxin-antitoxin system HicA family toxin	Toxin
HWQ66_RS06110	1327405..1327815	+	411	WP_000499744.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
HWQ66_RS06115	1327887..1329851	-	1965	Protein_1179	glycoside hydrolase family 127 protein	-
HWQ66_RS06120	1329862..1331262	-	1401	WP_000204802.1	MFS transporter	-
HWQ66_RS06125	1331488..1332303	+	816	WP_000891825.1	AraC family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-129)

Sequences

Toxin

Protein

• Length: 62 a.a.
• Molecular weight: 6800.89 Da
• Isoelectric Point: 11.7053

>T166261 WP_000833473.1 NZ_CP058223:1327203-1327388 [Escherichia coli]
VKSADVIAILKQHGWEHIRTRGSHHQFRHPVHPGLVTVPHPKKDIKPGTLAQIGRQAGLTF

Nucleotide

• Length: 186 bp

>T166261 NZ_CP074098:c1627072-1626788 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGACTTATAAGCTGGCATTTAACGAATCAGCACTAAAAGAGTGGAAAAAACTGGGTCATACCATACAGGAACAGTTTAA
AAAAAAGCTAAGGGAACGGCTTGAAAACCCTCGGGTTCCGGCTTCTCAATTACATGGAAGAAAGGATCAGTACAAAATTA
AGCTGCGTGGTGCAGGATACAGACTGGTGTACAGCGTCGAAGATGAAATTATCACGGTAACTGTCATTGGTGTTGGAAAG
CGTGAAAATGATGCCGTCTACAAAGTGACACGACACAGAAGCTAA

Antitoxin

Protein

• Length: 137 a.a.
• Molecular weight: 15241.15 Da
• Isoelectric Point: 4.5486

>AT166261 WP_000499744.1 NZ_CP058223:1327405-1327815 [Escherichia coli]
MFYPAYIHSDPDGSASGFFPDVPGCYFAGDTLDNAFQDARSALVAHFETLCEMNEELPLPGSVETHLVQRAQDFIGGQWL
LVDINMNQFEGRAERINITMPKRLLNKIDTYVRNNPDYANRSAFLAEAARRVLPGV

Nucleotide

• Length: 411 bp

>AT166261 NZ_CP074098:c1627310-1627062 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGGCATTTCAAATTTTAACGACTACTGCAGCAAGCATTACAGAACTGAAACGTGACCCAATGGGAACTTTCAATGCCGG
AGATGGCGCTCCAGTTGCCATTCTTAACCGTAATGAACCGGCTTTCTACTGTGTGCCTCCGGCACTTTATGCTCATCTTA
TGGATATTCTTGAGGATGAAGAACTCGGTCGTATTATCGATGAGCGTGCGAATGAACGCGTCATTGAGGTCAACATTGAT
GACTTATAA

Structures

Toxin

• AlphaFold DB: U9YXE2

Antitoxin

• AlphaFold DB: L4IWR9