Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4712516..4713013 Replicon chromosome
Accession NZ_CP058222
Organism Escherichia coli strain CFT073

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag HW370_RS22910 Protein ID WP_001173343.1
Coordinates 4712516..4712689 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID S1NQ59
Locus tag HW370_RS22915 Protein ID WP_001312331.1
Coordinates 4712717..4713013 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HW370_RS22900 (4709186) 4709186..4710643 + 1458 WP_000856826.1 dipeptide/tripeptide permease DtpC -
HW370_RS22905 (4710880) 4710880..4712397 + 1518 WP_000003806.1 lysine--tRNA ligase -
HW370_RS22910 (4712516) 4712516..4712689 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
HW370_RS22915 (4712717) 4712717..4713013 - 297 WP_001312331.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
HW370_RS22920 (4713242) 4713242..4713514 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
HW370_RS22925 (4713526) 4713526..4713756 - 231 WP_000371715.1 4Fe-4S mono-cluster protein YjdI -
HW370_RS22930 (4713937) 4713937..4715568 + 1632 WP_001216466.1 sensor histidine kinase -
HW370_RS22935 (4715565) 4715565..4716284 + 720 WP_000611291.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T166255 WP_001173343.1 NZ_CP058222:c4712689-4712516 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T166255 NZ_CP074096:4692312-4692596 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGACTTATGAACTGGAATTCGACCCGAGGGCCTTAAAAGAGTGGCATAAGCTGGGCGATACGGTGAAGGCTCAGCTTAA
GAAAAAGTTGGCTGATGTGCTATTGAACCCCAGAATCGACTCTGCCCGTTTAAACGGTCTTCCTGACTGCTATAAAATTA
AGCTTAAATCGTCCGGTTATCGTCTGGTGTACCAGGTTCGGGATGACGTTGTGATTGTGTTTGTTGTTGCGGTCGGCAAG
CGAGAACATTCAGCCGTCTATCACGATGCAAACAAACGGCTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11440.95 Da        Isoelectric Point: 4.1705

>AT166255 WP_001312331.1 NZ_CP058222:c4713013-4712717 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLSIMTVDDYFRQFYK

Download         Length: 297 bp

>AT166255 NZ_CP074096:4692080-4692322 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGGCCACGCTGAACGTCCGTCTGGATGACAAACTCAAAAATGAGGCTTATGCCGTGCTGGAAAAACTGAACATTACTCC
AACGGAAGCCGTGCGATTACTGTTCCAGTACGTCGCGGAAACGGGGCGCATGCCAGTGAAAACCGTCACTCTTAGCGACA
GTGAAGATGCATTGATTCAGACAGTTCGGGAGAGGCTGTCTAGTCCGCAAAAGGGAATCAAGGTTAGTCTGGATGACTTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB S1NQ59

References