Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 4337499..4338049 Replicon chromosome
Accession NZ_CP058222
Organism Escherichia coli strain CFT073

Toxin (Protein)


Gene name ccdB Uniprot ID S1PDG4
Locus tag HW370_RS21100 Protein ID WP_001160966.1
Coordinates 4337499..4337813 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag HW370_RS21105 Protein ID WP_000125566.1
Coordinates 4337816..4338049 (-) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HW370_RS21075 (4333174) 4333174..4334460 + 1287 WP_000800457.1 peptidylprolyl isomerase SurA -
HW370_RS21080 (4334460) 4334460..4335449 + 990 WP_000241229.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
HW370_RS21085 (4335446) 4335446..4336267 + 822 WP_001065363.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
HW370_RS21090 (4336270) 4336270..4336647 + 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
HW370_RS21095 (4336654) 4336654..4337502 + 849 WP_000257182.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
HW370_RS21100 (4337499) 4337499..4337813 - 315 WP_001160966.1 CcdB family protein Toxin
HW370_RS21105 (4337816) 4337816..4338049 - 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
HW370_RS21110 (4338135) 4338135..4338614 - 480 WP_000624375.1 type 3 dihydrofolate reductase -
HW370_RS21115 (4338806) 4338806..4340668 - 1863 WP_000377123.1 glutathione-regulated potassium-efflux system protein KefC -
HW370_RS21120 (4340661) 4340661..4341191 - 531 WP_000600725.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
HW370_RS21125 (4341299) 4341299..4342630 - 1332 WP_001183207.1 MFS transporter -
HW370_RS21130 (4342689) 4342689..4342976 - 288 WP_000203741.1 ferredoxin-like protein FixX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11711.72 Da        Isoelectric Point: 9.0851

>T166247 WP_001160966.1 NZ_CP058222:c4337813-4337499 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T166247 NZ_CP074096:3047954-3048481 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGATGTTTACAGACTGGCATGAGGCCGCGATAGGGAAAACCCACAATCGAATGAATTTTGATTGTGGGGATGCCGATCT
GAACCAATTCCTGCAACGCCATGCACGACAAAATCATGAGAAAGGGACAACGAAAACCTATGTTGCGCTTGATAATTCGG
ATGTTACACGTATCCATGGATTTTACTCAGTCAGTCCTGCATCACTGATATATGCACAGGTTCCCGGCGCAATCAGCAAA
GGATTAGGGAGACACGATGTGCCAGTGTTTCGTCTGGGTCGTTTAGCCGTAGACAAATCTATGCAGGGGCAAGGGCTGGG
AGCACAACTTTTGTTATCTGCCGGAAAGCGTTGCATACAGGCGGCTTTGCAGGTCGGTGGCGTAGCCCTGCTTATTGATG
CCAAAAATAAACAGGTCTGCGACTGGTACAAAGGATTTGGCGCAGTACCATTAAACGATCAACCCCTTTCCTTGTTGCTG
TCGTTTAAAACGCTTTATGCGGCTTTATCTGCATCTGGTAGGTTATGA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT166247 WP_000125566.1 NZ_CP058222:c4338049-4337816 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT166247 NZ_CP074096:3047667-3047957 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGAAAACCATGCCTCAGATAGCTATAGAATCTAACGAAAGGCTGTCGTTACGTGTATCGACGGATGCCAAAAAGTTGAT
AGTCCGCGCTGCGGCAATCCAGCAAACCAACCTGACGGATTTTGTGGTCAGTAACGTACTACCTGTCGCACAGAAGATTG
TTGATGCCGCCGAACGGGTATATCTGACGGAGCGTGATACTCAAATGATCATGGAAATTTTAGATAACCCGCCAGCGCCG
AATGAAAAGCTTCTTGCTGCTGCTTTCGCCCTGCCGGACATGAAGAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G815

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References