Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3832790..3833408 Replicon chromosome
Accession NZ_CP058222
Organism Escherichia coli strain CFT073

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag HW370_RS18760 Protein ID WP_001291435.1
Coordinates 3833190..3833408 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag HW370_RS18755 Protein ID WP_000344800.1
Coordinates 3832790..3833164 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HW370_RS18745 (3827880) 3827880..3829073 + 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
HW370_RS18750 (3829096) 3829096..3832245 + 3150 WP_001132480.1 efflux RND transporter permease AcrB -
HW370_RS18755 (3832790) 3832790..3833164 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
HW370_RS18760 (3833190) 3833190..3833408 + 219 WP_001291435.1 HHA domain-containing protein Toxin
HW370_RS18765 (3833581) 3833581..3834132 + 552 WP_000102539.1 maltose O-acetyltransferase -
HW370_RS18770 (3834248) 3834248..3834718 + 471 WP_001304825.1 YlaC family protein -
HW370_RS18775 (3834882) 3834882..3836432 + 1551 WP_001528761.1 cyclic-guanylate-specific phosphodiesterase PdeB -
HW370_RS18780 (3836474) 3836474..3836827 - 354 WP_000878140.1 DUF1428 domain-containing protein -
HW370_RS18790 (3837206) 3837206..3837517 + 312 WP_000409908.1 MGMT family protein -
HW370_RS18795 (3837548) 3837548..3838120 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T166245 WP_001291435.1 NZ_CP058222:3833190-3833408 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T166245 NZ_CP074096:c1626993-1626709 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGACTTATAAGCTGGCATTTAACGAATCAGCACTAAAAGAGTGGAAAAAACTGGGTCATACCATACAGGAACAGTTTAA
AAAAAAGCTAAGGGAACGGCTTGAAAACCCTCGGGTTCCGGCTTCTCAATTACATGGAAGAAAGGATCAGTACAAAATTA
AGCTGCGTGGTGCAGGATACAGACTGGTGTACAGCGTCGAAGATGAAATTATCACGGTAACTGTCATTGGTGTTGGAAAG
CGTGAAAATGATGCCGTCTACAAAGTGACACGACACAGAAGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT166245 WP_000344800.1 NZ_CP058222:3832790-3833164 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT166245 NZ_CP074096:c1627231-1626983 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGGCATTTCAAATTTTAACGACTACTGCAGCAAGCATTACAGAACTGAAACGTGACCCAATGGGAACTTTCAATGCCGG
AGATGGCGCTCCAGTTGCCATTCTTAACCGTAATGAACCGGCTTTCTACTGTGTGCCTCCGGCACTTTATGCTCATCTTA
TGGATATTCTTGAGGATGAAGAACTCGGTCGTATTATCGATGAGCGTGCGAATGAACGCGTCATTGAGGTCAACATTGAT
GACTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References