Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 2018355..2019186 Replicon chromosome
Accession NZ_CP058222
Organism Escherichia coli strain CFT073

Toxin (Protein)


Gene name yeeV Uniprot ID S1P968
Locus tag HW370_RS09640 Protein ID WP_000854814.1
Coordinates 2018355..2018729 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID S1PWQ3
Locus tag HW370_RS09645 Protein ID WP_001285586.1
Coordinates 2018818..2019186 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HW370_RS09605 (2013751) 2013751..2014917 + 1167 WP_001297905.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -
HW370_RS09610 (2015036) 2015036..2015509 + 474 WP_001105376.1 DNA gyrase inhibitor SbmC -
HW370_RS09615 (2015707) 2015707..2016765 + 1059 WP_001200890.1 FUSC family protein -
HW370_RS09620 (2016937) 2016937..2017266 + 330 WP_000450409.1 DUF496 family protein -
HW370_RS09625 (2017367) 2017367..2017723 - 357 WP_000929389.1 EutP/PduV family microcompartment system protein -
HW370_RS09630 (2018038) 2018038..2018118 - 81 Protein_1891 hypothetical protein -
HW370_RS09635 (2018164) 2018164..2018358 - 195 WP_000988601.1 DUF5983 family protein -
HW370_RS09640 (2018355) 2018355..2018729 - 375 WP_000854814.1 type IV toxin-antitoxin system cytoskeleton-binding toxin CbtA Toxin
HW370_RS09645 (2018818) 2018818..2019186 - 369 WP_001285586.1 type IV toxin-antitoxin system cytoskeleton bundling-enhancing antitoxin CbeA Antitoxin
HW370_RS09650 (2019260) 2019260..2019481 - 222 WP_000692323.1 DUF987 domain-containing protein -
HW370_RS09655 (2019550) 2019550..2020026 - 477 WP_001351157.1 RadC family protein -
HW370_RS09660 (2020042) 2020042..2020527 - 486 WP_000213703.1 antirestriction protein -
HW370_RS09665 (2020618) 2020618..2021439 - 822 WP_001234569.1 DUF932 domain-containing protein -
HW370_RS09670 (2021660) 2021660..2022070 - 411 WP_000846704.1 hypothetical protein -
HW370_RS09675 (2022086) 2022086..2022763 - 678 WP_001362823.1 hypothetical protein -
HW370_RS09680 (2022899) 2022899..2023969 - 1071 WP_000102631.1 patatin-like phospholipase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13898.94 Da        Isoelectric Point: 7.8522

>T166234 WP_000854814.1 NZ_CP058222:c2018729-2018355 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T166234 NZ_CP074094:c3728613-3728245 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGACCCTACAACTTATCTCAGCGGAAGAGATAATTCAGTTTCACGACAGGCTTCTCCGCGTTACGCCTGGTGTAACAGG
CATGCCTGATCCTGGCCGCGCGGAAGCGCTAATGTACCGGGTACTCAAGCAAATCGAATATGAAGGGGTGACCGACGTGT
GGCTGCTGGCGGCAATGCATTTGCTCGCTATATCCCGTGGGCATATCTTCAATGATGGTAACAAACGTACCGCCTTATTT
ATTACGCTGCTGTTTTTAAAGCGTAACGGGATCTCACTCGCTGCGAATCCGGATTTTGTCGATATGACAGTCGATGCGGC
GGCAGGGCGGCTTACGCTGGAGCAAATTGCCGTTCGCTTACGTGCCTGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13563.41 Da        Isoelectric Point: 5.0468

>AT166234 WP_001285586.1 NZ_CP058222:c2019186-2018818 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELSPRYQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT166234 NZ_CP074094:c3728831-3728610 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGCGTACGGTTAACTATAGCGAAGCGCGGCAAAATCTGGCCGAAGTCCTGGAAAGTGCGGTGACGGGGGGGCCTGTTAC
CATCACGCGTCGTGGGCATAAGTCCGCAGTGATCATCAGCGCCGAGGAGTTTGAGCGTTATCAGACGGCGCGAATGGATG
ATGAGTTTGCTGCCATTATGGCGGTTCATGGCAATGAGCTCAGGGAGCTGGCGGATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FY50

Antitoxin

Source ID Structure
AlphaFold DB S1PWQ3

References