Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4607145..4607642 Replicon chromosome
Accession NZ_CP058220
Organism Escherichia coli strain 83972

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag HW371_RS22330 Protein ID WP_001173343.1
Coordinates 4607145..4607318 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID S1NQ59
Locus tag HW371_RS22335 Protein ID WP_001312331.1
Coordinates 4607346..4607642 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HW371_RS22320 (4603815) 4603815..4605272 + 1458 WP_000856826.1 dipeptide/tripeptide permease DtpC -
HW371_RS22325 (4605509) 4605509..4607026 + 1518 WP_000003806.1 lysine--tRNA ligase -
HW371_RS22330 (4607145) 4607145..4607318 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
HW371_RS22335 (4607346) 4607346..4607642 - 297 WP_001312331.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
HW371_RS22340 (4607871) 4607871..4608143 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
HW371_RS22345 (4608155) 4608155..4608385 - 231 WP_000371715.1 4Fe-4S mono-cluster protein YjdI -
HW371_RS22350 (4608566) 4608566..4610197 + 1632 WP_001216466.1 sensor histidine kinase -
HW371_RS22355 (4610194) 4610194..4610913 + 720 WP_000611291.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T166226 WP_001173343.1 NZ_CP058220:c4607318-4607145 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T166226 NZ_CP074093:c86967-86569 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGCTGAAGTTTATGCTGGATACTAACATCTGCATTTTCACGATAAAAAACAAACCTGCCAGCGTCAGGGAGCGTTTTAA
CCTGAACCAGGGGAGAATGTGTATCAGCTCGGTCACCCTGATGGAGCTGATTTACGGCGCCGAAAAAAGCCAGATGCCCG
AAAGAAATCTCGCTGTGATAGAGGGGTTTGTTTCCCGACTTGATGTTCTGGATTATGACACGCCGGCCGCCACCCATACG
GGGCAGATCAGGGCAGAGCTTGCCCGTCAGGGACGCCCTGTCGGGCCATTCGATCAGATGATTGCCGGTCACGCACGCAG
CCGGGGACTGATTGTTGTCACGAACAACACCCGGGAGTTTGAACGTGTGGGTGGACTGAGAACTGAGGACTGGAGCTGA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11440.95 Da        Isoelectric Point: 4.1705

>AT166226 WP_001312331.1 NZ_CP058220:c4607642-4607346 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLSIMTVDDYFRQFYK

Download         Length: 297 bp

>AT166226 NZ_CP074093:c87194-86967 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGGAAACCAGCGTATTTCTCAGTAACCGCAGCCAGGCGGTCAGACTGCCAAAAGCAGTGGCTTTGCCGGAAAATGTAAA
ACGTGTGGAAGTTATCGCTGTCGGGCGAACCCGAATTATCACGCCGGCCGGAGAATCGTGGGATGAATGGTTCGACGGGA
ACAACGTCAGCGCCGATTTTATGGATAACCGGGAACAACCCTCTGTGCAGGAACGGGAATCATTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB S1NQ59

References