Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 4201340..4201890 Replicon chromosome
Accession NZ_CP058220
Organism Escherichia coli strain 83972

Toxin (Protein)


Gene name ccdB Uniprot ID S1PDG4
Locus tag HW371_RS20355 Protein ID WP_001160966.1
Coordinates 4201340..4201654 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag HW371_RS20360 Protein ID WP_000125566.1
Coordinates 4201657..4201890 (-) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HW371_RS20330 (4197015) 4197015..4198301 + 1287 WP_000800457.1 peptidylprolyl isomerase SurA -
HW371_RS20335 (4198301) 4198301..4199290 + 990 WP_000241229.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
HW371_RS20340 (4199287) 4199287..4200108 + 822 WP_001065363.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
HW371_RS20345 (4200111) 4200111..4200488 + 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
HW371_RS20350 (4200495) 4200495..4201343 + 849 WP_000257182.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
HW371_RS20355 (4201340) 4201340..4201654 - 315 WP_001160966.1 CcdB family protein Toxin
HW371_RS20360 (4201657) 4201657..4201890 - 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
HW371_RS20365 (4201976) 4201976..4202455 - 480 WP_000624375.1 type 3 dihydrofolate reductase -
HW371_RS20370 (4202647) 4202647..4204509 - 1863 WP_000377123.1 glutathione-regulated potassium-efflux system protein KefC -
HW371_RS20375 (4204502) 4204502..4205032 - 531 WP_000600725.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
HW371_RS20380 (4205140) 4205140..4206471 - 1332 WP_001183207.1 MFS transporter -
HW371_RS20385 (4206530) 4206530..4206817 - 288 WP_000203741.1 ferredoxin-like protein FixX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11711.72 Da        Isoelectric Point: 9.0851

>T166220 WP_001160966.1 NZ_CP058220:c4201654-4201340 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T166220 NZ_CP074092:c4240416-4240105 [Salmonella enterica subsp. enterica serovar Typhimurium]
GTGCATGTTATCAGCCGAAAACCGTTCAATGAAGCGATGCTCATGTATCCAAACCATGAGCTTGCTCTCACTGAGTTGCT
GAATGTTCTGGAGAAAAAAACATTCACTCAGCCTGAAGAGATGAAGCGGTATATTCCTTCACTGGATAATTTTAAGTACA
GAGATAAATGGTGGGTGATTGATGTTTCTGGTAACAGCCTGAGACTCATTTCCTACATAGATTTCAGGTTACATAAGATA
TTTGTGAAACATATCGTTTCCCATGCCGAATATGACAAACTGACCGCATACTATCGGGGTAATAAAGAATGA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT166220 WP_000125566.1 NZ_CP058220:c4201890-4201657 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT166220 NZ_CP074092:c4240108-4239662 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGAGAACTCATCGTCAGATGGATGCAACCAGCGCAAAAAAGATCGTTGATACCTTCAGCGATGCGGTAAAAACCGTCCC
ACTGATGGGGGAAGACCGAAATGACAATGAGTATCGCAGGGCACTAGCGCTAGTGGAGTTTCTGGTCGACCACGACGATC
TTGAAAACCCACTATTTGAATTGCTCTGTGCCCGAATCAGTGAATACGAAAAACATGCGCCGGAATTCAAAGCACTCAAC
CAACACCTGGAGAAAACGCCCCCTGGCGTTTCAGTATTGCGAACGCTCATGGATCAATACGGTCTCAAAGCAGCAGATCT
TGCCAACGAACTTGGTTCTAAATCGAACGTCAGCAACATCTTAAATGGCCGCAGAGCACTAACGGTTAATCATATTAAAG
CGCTTACACAACGCTTCAAACTACCAGCAGATGCCTTCATCGAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G815

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References