Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3689846..3690464 Replicon chromosome
Accession NZ_CP058220
Organism Escherichia coli strain 83972

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag HW371_RS17940 Protein ID WP_001291435.1
Coordinates 3690246..3690464 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag HW371_RS17935 Protein ID WP_000344800.1
Coordinates 3689846..3690220 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HW371_RS17925 (3684936) 3684936..3686129 + 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
HW371_RS17930 (3686152) 3686152..3689301 + 3150 WP_001132480.1 efflux RND transporter permease AcrB -
HW371_RS17935 (3689846) 3689846..3690220 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
HW371_RS17940 (3690246) 3690246..3690464 + 219 WP_001291435.1 HHA domain-containing protein Toxin
HW371_RS17945 (3690637) 3690637..3691188 + 552 WP_000102539.1 maltose O-acetyltransferase -
HW371_RS17950 (3691304) 3691304..3691774 + 471 WP_001304825.1 YlaC family protein -
HW371_RS17955 (3691938) 3691938..3693488 + 1551 WP_001528761.1 cyclic-guanylate-specific phosphodiesterase PdeB -
HW371_RS17960 (3693530) 3693530..3693883 - 354 WP_000878140.1 DUF1428 domain-containing protein -
HW371_RS17970 (3694262) 3694262..3694573 + 312 WP_000409908.1 MGMT family protein -
HW371_RS17975 (3694604) 3694604..3695176 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T166218 WP_001291435.1 NZ_CP058220:3690246-3690464 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T166218 NZ_CP074092:c3728316-3727948 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGACCCTACAACTTATCTCAGCGGAAGAGATAATTCAGTTTCACGACAGGCTTCTCCGCGTTACGCCTGGTGTAACAGG
CATGCCTGATCCTGGCCGCGCGGAAGCGCTAATGTACCGGGTACTCAAGCAAATCGAATATGAAGGGGTGACCGACGTGT
GGCTGCTGGCGGCAATGCATTTGCTCGCTATATCCCGTGGGCATATCTTCAATGATGGTAACAAACGTACCGCCTTATTT
ATTACGCTGCTGTTTTTAAAGCGTAACGGGATCTCACTCGCTGCGAATCCGGATTTTGTCGATATGACAGTCGATGCGGC
GGCAGGGCGGCTTACGCTGGAGCAAATTGCCGTTCGCTTACGTGCCTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT166218 WP_000344800.1 NZ_CP058220:3689846-3690220 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT166218 NZ_CP074092:c3728534-3728313 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGCGTACGGTTAACTATAGCGAAGCGCGGCAAAATCTGGCCGAAGTCCTGGAAAGTGCGGTGACGGGGGGGCCTGTTAC
CATCACGCGTCGTGGGCATAAGTCCGCAGTGATCATCAGCGCCGAGGAGTTTGAGCGTTATCAGACGGCGCGAATGGATG
ATGAGTTTGCTGCCATTATGGCGGTTCATGGCAATGAGCTCAGGGAGCTGGCGGATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References