Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 995625..996279 Replicon chromosome
Accession NZ_CP058220
Organism Escherichia coli strain 83972

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag HW371_RS04935 Protein ID WP_000244781.1
Coordinates 995872..996279 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag HW371_RS04930 Protein ID WP_000354046.1
Coordinates 995625..995891 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HW371_RS04910 (991713) 991713..993146 - 1434 WP_001350137.1 6-phospho-beta-glucosidase BglA -
HW371_RS04915 (993191) 993191..993502 + 312 WP_001182956.1 N(4)-acetylcytidine aminohydrolase -
HW371_RS04920 (993666) 993666..994325 + 660 WP_000250274.1 hemolysin III family protein -
HW371_RS04925 (994402) 994402..995382 - 981 WP_000886079.1 tRNA-modifying protein YgfZ -
HW371_RS04930 (995625) 995625..995891 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
HW371_RS04935 (995872) 995872..996279 + 408 WP_000244781.1 protein YgfX Toxin
HW371_RS04940 (996319) 996319..996840 - 522 WP_001055874.1 flavodoxin FldB -
HW371_RS04945 (996952) 996952..997848 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
HW371_RS04950 (997873) 997873..998583 + 711 WP_000715230.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
HW371_RS04955 (998589) 998589..1000322 + 1734 WP_000813189.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T166203 WP_000244781.1 NZ_CP058220:995872-996279 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T166203 NZ_CP074087:c4798862-4798578 [Klebsiella pneumoniae subsp. pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCAGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGACCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT166203 WP_000354046.1 NZ_CP058220:995625-995891 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT166203 NZ_CP074087:c4799094-4798852 [Klebsiella pneumoniae subsp. pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCAGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References