Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | ccdAB/CcdA(antitoxin) |
| Location | 4152167..4152717 | Replicon | chromosome |
| Accession | NZ_CP058217 | ||
| Organism | Escherichia coli Nissle 1917 | ||
Toxin (Protein)
| Gene name | ccdB | Uniprot ID | S1PDG4 |
| Locus tag | HW372_RS19890 | Protein ID | WP_001160966.1 |
| Coordinates | 4152167..4152481 (-) | Length | 105 a.a. |
Antitoxin (Protein)
| Gene name | ccdA | Uniprot ID | V0VEX8 |
| Locus tag | HW372_RS19895 | Protein ID | WP_000125566.1 |
| Coordinates | 4152484..4152717 (-) | Length | 78 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| HW372_RS19865 (4147842) | 4147842..4149128 | + | 1287 | WP_000800457.1 | peptidylprolyl isomerase SurA | - |
| HW372_RS19870 (4149128) | 4149128..4150117 | + | 990 | WP_000241229.1 | 4-hydroxythreonine-4-phosphate dehydrogenase PdxA | - |
| HW372_RS19875 (4150114) | 4150114..4150935 | + | 822 | WP_001065363.1 | 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA | - |
| HW372_RS19880 (4150938) | 4150938..4151315 | + | 378 | WP_000610901.1 | Co2+/Mg2+ efflux protein ApaG | - |
| HW372_RS19885 (4151322) | 4151322..4152170 | + | 849 | WP_000257182.1 | bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH | - |
| HW372_RS19890 (4152167) | 4152167..4152481 | - | 315 | WP_001160966.1 | CcdB family protein | Toxin |
| HW372_RS19895 (4152484) | 4152484..4152717 | - | 234 | WP_000125566.1 | type II toxin-antitoxin system CcdA family antitoxin | Antitoxin |
| HW372_RS19900 (4152803) | 4152803..4153282 | - | 480 | WP_000624375.1 | type 3 dihydrofolate reductase | - |
| HW372_RS19905 (4153474) | 4153474..4155336 | - | 1863 | WP_000377123.1 | glutathione-regulated potassium-efflux system protein KefC | - |
| HW372_RS19910 (4155329) | 4155329..4155859 | - | 531 | WP_000600725.1 | glutathione-regulated potassium-efflux system oxidoreductase KefF | - |
| HW372_RS19915 (4155967) | 4155967..4157298 | - | 1332 | WP_001183207.1 | MFS transporter | - |
| HW372_RS19920 (4157357) | 4157357..4157644 | - | 288 | WP_000203741.1 | ferredoxin-like protein FixX | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 105 a.a. Molecular weight: 11711.72 Da Isoelectric Point: 9.0851
>T166193 WP_001160966.1 NZ_CP058217:c4152481-4152167 [Escherichia coli Nissle 1917]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI
Download Length: 315 bp
>T166193 NZ_CP074076:4888278-4888607 [Xanthomonas oryzae]
TTGCATCATGTCGCCGAACGCCGATGGCCGTTTCTGTTTACTGACAGCCACGCCTACTGCCAGTTGGCGAGCTTCTATTC
CGATTTATCTGATCTCGACAAGATCGACTGGCCGTTGCTCCAAGCACGCGATTTCCGGCGCGACCCAGACGACCCAGCCA
AGTTCGAGCGCTATCAGGCCGAGGCCCTGATTCACCGACACTTGCCGCTTGATGGCCTGCGCGGCATTGTGTGTCATACG
GATGGTTTGAAACAGACCATCGAGCGGCAACTCCAAGAGCGAAACCTGAAATTGCCGGTTCATACCCGCACCGATTGGTA
TTTCCGATGA
TTGCATCATGTCGCCGAACGCCGATGGCCGTTTCTGTTTACTGACAGCCACGCCTACTGCCAGTTGGCGAGCTTCTATTC
CGATTTATCTGATCTCGACAAGATCGACTGGCCGTTGCTCCAAGCACGCGATTTCCGGCGCGACCCAGACGACCCAGCCA
AGTTCGAGCGCTATCAGGCCGAGGCCCTGATTCACCGACACTTGCCGCTTGATGGCCTGCGCGGCATTGTGTGTCATACG
GATGGTTTGAAACAGACCATCGAGCGGCAACTCCAAGAGCGAAACCTGAAATTGCCGGTTCATACCCGCACCGATTGGTA
TTTCCGATGA
Antitoxin
Download Length: 78 a.a. Molecular weight: 8530.53 Da Isoelectric Point: 5.8062
>AT166193 WP_000125566.1 NZ_CP058217:c4152717-4152484 [Escherichia coli Nissle 1917]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF
Download Length: 234 bp
>AT166193 NZ_CP074076:4888604-4889023 [Xanthomonas oryzae]
ATGATCACGTTTACACAAGGCAATCTACTGGAGTCTGGCGCAGAGGCGCTGGTCAACACCGTCAACACCGTCGGCGTGAT
GGGCAAGGGCATCGCGCTGATGTTCAAGGAGCGCTTCAAGGAGAACTTCCTCCGATATGCCGCCGCCTGCAAAGACAACC
AAGTGCGCATTGGCAAAATTTTCGTCACCGAAGTCAACGAACTGGACGGGCCACGCTGGATCATCAATTTTCCGACCAAA
CAGCACTGGCGTGGCGATTCCCGCATCGAATGGATCACTGAAGGATTGCAGGACTTGCACCGTTTTCTGATCGAGAACAA
AGTCAAATCCATTGCCATCCCGCCACTGGGCGCGGGCAATGGAGGATTGGACTGGGCCGAGGTGCGCCCACTGATCGAAG
AAGTACTAGGGAACCTCTGA
ATGATCACGTTTACACAAGGCAATCTACTGGAGTCTGGCGCAGAGGCGCTGGTCAACACCGTCAACACCGTCGGCGTGAT
GGGCAAGGGCATCGCGCTGATGTTCAAGGAGCGCTTCAAGGAGAACTTCCTCCGATATGCCGCCGCCTGCAAAGACAACC
AAGTGCGCATTGGCAAAATTTTCGTCACCGAAGTCAACGAACTGGACGGGCCACGCTGGATCATCAATTTTCCGACCAAA
CAGCACTGGCGTGGCGATTCCCGCATCGAATGGATCACTGAAGGATTGCAGGACTTGCACCGTTTTCTGATCGAGAACAA
AGTCAAATCCATTGCCATCCCGCCACTGGGCGCGGGCAATGGAGGATTGGACTGGGCCGAGGTGCGCCCACTGATCGAAG
AAGTACTAGGGAACCTCTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A829G815 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A7U9LTM9 |