Detailed information of TA system TA166192

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3952921..3953719
• Replicon: chromosome
• Accession: NZ_CP058217
• Organism: Escherichia coli Nissle 1917

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: Q8VRA6
• Locus tag: HW372_RS18935
• Protein ID: WP_000854730.1
• Coordinates: 3953342..3953719 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A0P0SQV8
• Locus tag: HW372_RS18930
• Protein ID: WP_001285481.1
• Coordinates: 3952921..3953295 (+)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HW372_RS18890 (3948896)	3948896..3949348	+	453	WP_000682723.1	hypothetical protein	-
HW372_RS18895 (3949466)	3949466..3949699	+	234	WP_001213776.1	DUF905 family protein	-
HW372_RS18900 (3949799)	3949799..3950620	+	822	WP_024199417.1	DUF932 domain-containing protein	-
HW372_RS18905 (3950620)	3950620..3950865	+	246	WP_001164966.1	hypothetical protein	-
HW372_RS18910 (3950959)	3950959..3951432	+	474	WP_001313575.1	antirestriction protein	-
HW372_RS18915 (3951448)	3951448..3951924	+	477	WP_001313574.1	RadC family protein	-
HW372_RS18920 (3951987)	3951987..3952208	+	222	WP_000692315.1	DUF987 domain-containing protein	-
HW372_RS18925 (3952227)	3952227..3952871	+	645	WP_000086759.1	hypothetical protein	-
HW372_RS18930 (3952921)	3952921..3953295	+	375	WP_001285481.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HW372_RS18935 (3953342)	3953342..3953719	+	378	WP_000854730.1	TA system toxin CbtA family protein	Toxin
HW372_RS18940 (3953716)	3953716..3954208	+	493	Protein_3712	DUF5983 family protein	-
HW372_RS18945 (3954287)	3954287..3955275	-	989	Protein_3713	IS630 family transposase	-
HW372_RS18950 (3955423)	3955423..3955611	-	189	Protein_3714	IS66 family transposase	-
HW372_RS18960 (3955784)	3955784..3956917	+	1134	WP_000555401.1	IS110-like element ISEc45 family transposase	-
HW372_RS18965 (3957171)	3957171..3958575	-	1405	Protein_3716	IS66 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14212.21 Da
• Isoelectric Point: 7.2923

>T166192 WP_000854730.1 NZ_CP058217:3953342-3953719 [Escherichia coli Nissle 1917]
MKTLPDTHVREASRCPSPVTIWQTLLTQLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRSNYRMVNDIIRGEHSEAKR

Nucleotide

• Length: 378 bp

>T166192 NZ_CP074076:3085312-3085563 [Xanthomonas oryzae]
ATGCTACGGCTGCGTGAATTCCTGAGGCCGAAGAATCCCGATGCCGCGAGGCGGGCCGGCGAAGCGATTAGGCGCGGCGT
CCAGGCTCTCGGTGCCCACCCTCGAATGGGGCGGCTCATAGAAGATCTGTCTGAACAGTATCGGGAGTGGCTTATCGATT
TTGGGGATAGCGGCTATGTGGCTCGGTACCGCTTCGATGGGGATACCGTCACGATTTTGGCCGTGCGACACCAGAAGGAA
GCGGGGTACTGA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 13817.53 Da
• Isoelectric Point: 4.7511

>AT166192 WP_001285481.1 NZ_CP058217:3952921-3953295 [Escherichia coli Nissle 1917]
VSDTLHETNYPDDNNDRPWWGLPCTVTPCFGARLVQEGNQLHYLADRAGIRGQFSDADAYHLDQAFPLLMKQLELMLTIG
ELNPRHQHTVTLYARGLTCEADTLGSCGYVYMAVYPTLAPATTS

Nucleotide

• Length: 375 bp

>AT166192 NZ_CP074076:3085007-3085285 [Xanthomonas oryzae]
ATGGTTACGGCAACTTCCATCAAGCTTGATGATGAATTGAAAGGGCGGGTTCAGCACTTGGCAGAGTCGCGCCGGCGTAC
CCCGCATTGGATCATGCGCGAAGCCATTGAGCAGTACGTCGAGCGCGAGGAAAAGCGCGAGGCGCTGAACCGGGACACGC
TCAAGGCATGGGATGAATTTCAGGCGACTGGCCTGCATGTGACCGCTGAGGAAGTGGACAAGTGGCTCGCTAGCTGGGGA
ACTGACGACGAACTGCCTCCGCCAGAATGCCACAAGTAA

Structures

Toxin

• AlphaFold DB: A0A0H2V671

Antitoxin

• AlphaFold DB: A0A0P0SQV8