Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 3014914..3015715 Replicon chromosome
Accession NZ_CP058217
Organism Escherichia coli Nissle 1917

Toxin (Protein)


Gene name yeeV Uniprot ID A0A0H2V687
Locus tag HW372_RS14520 Protein ID WP_000854739.1
Coordinates 3014914..3015294 (-) Length 127 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A0P7R0L9
Locus tag HW372_RS14525 Protein ID WP_001285482.1
Coordinates 3015341..3015715 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HW372_RS14485 (3010505) 3010505..3011059 - 555 WP_001001909.1 molecular chaperone YcdY -
HW372_RS14490 (3011083) 3011083..3011820 - 738 WP_000283664.1 zinc-binding phosphatase -
HW372_RS14495 (3011875) 3011875..3012813 - 939 WP_000351287.1 glyoxylate/hydroxypyruvate reductase GhrA -
HW372_RS14505 (3013284) 3013284..3014127 - 844 Protein_2846 DUF4942 domain-containing protein -
HW372_RS14510 (3014212) 3014212..3014409 - 198 WP_000772027.1 DUF957 domain-containing protein -
HW372_RS14515 (3014429) 3014429..3014917 - 489 WP_001054232.1 DUF5983 family protein -
HW372_RS14520 (3014914) 3014914..3015294 - 381 WP_000854739.1 TA system toxin CbtA family protein Toxin
HW372_RS14525 (3015341) 3015341..3015715 - 375 WP_001285482.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
HW372_RS14530 (3015765) 3015765..3016409 - 645 WP_000094915.1 hypothetical protein -
HW372_RS14535 (3016428) 3016428..3016649 - 222 WP_000692312.1 DUF987 domain-containing protein -
HW372_RS14540 (3016712) 3016712..3017188 - 477 WP_001186170.1 RadC family protein -
HW372_RS14545 (3017204) 3017204..3017677 - 474 WP_000855076.1 antirestriction protein -
HW372_RS14550 (3017940) 3017940..3018761 - 822 WP_001234710.1 DUF932 domain-containing protein -
HW372_RS14555 (3018940) 3018940..3019029 - 90 WP_230607454.1 DUF905 family protein -
HW372_RS14560 (3019172) 3019172..3019627 - 456 WP_000581502.1 IrmA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 14214.14 Da        Isoelectric Point: 6.4773

>T166190 WP_000854739.1 NZ_CP058217:c3015294-3014914 [Escherichia coli Nissle 1917]
MKTLPDTHVREASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGVPSQLINSIDILRARRATGLMTRDNYRTVNDITLGRHPEEAKQ

Download         Length: 381 bp

>T166190 NZ_CP074076:1232841-1233122 [Xanthomonas oryzae]
ATGATCAGGAGCTTTATCGACAAGGACGCCGAAAAGATCTGGCTGGGTGAGCGTTCCCGCCGGTTGCCTGCCGACATCCA
ATTGGTCGCGCGCCGCAAGTTGCGCATGCTCAATGCCGCCGCGCATCTCGACGATCTGCGGATCCCGCCCGCCAACCGGC
TGGAAGCGTTGAAGGGCCAGCAGCGCGGCCAGTACAGCATCCGGATCAACGATCAATGGCGCATCTGCTTCCGATGGATG
GAAGGCGATGTGGTCCAGGTCGAAATCGTCGATTACCACTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 13791.44 Da        Isoelectric Point: 4.7511

>AT166190 WP_001285482.1 NZ_CP058217:c3015715-3015341 [Escherichia coli Nissle 1917]
VSDTLHETNYPDDNNDRPWWGLPCTVTPCFGARLVQEGNQLHYLADRAGIRGQFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYARGLTCEADTLGSCGYVYMAVYPTLAPATTS

Download         Length: 375 bp

>AT166190 NZ_CP074076:1233140-1233430 [Xanthomonas oryzae]
ATGACGGTCCTACCCAATATCCATCCTGGCGACATTCTGCTTGAGGAGTTTCTCGAGCCTCTGGGCATCAGCCAGAACGC
ACTGGCACGCGCCACCGGCGTCCCGCCGCGCCGTATCAACGAAATCGTGCTGGGCAAGCGCGGCATCACTGCCGACACTG
CCGTGCGTCTGGCCGCCGCGTTGGGCACCTCCGAGCGCTTCTGGCTGGGCCTGCAGGCCGACTACGAGCTGGAACAGGCG
CATCGCGCGCTGGGCGATTTGCCGGCACGGATCAAGCGGCTGGCGGCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H2V687

Antitoxin

Source ID Structure
AlphaFold DB A0A0P7R0L9

References