Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 2538341..2539929 Replicon chromosome
Accession NZ_CP058217
Organism Escherichia coli Nissle 1917

Toxin (Protein)


Gene name hipA Uniprot ID A0A1L1KDK2
Locus tag HW372_RS12090 Protein ID WP_001125431.1
Coordinates 2538607..2539929 (+) Length 441 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID V0VAH2
Locus tag HW372_RS12085 Protein ID WP_001304368.1
Coordinates 2538341..2538607 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HW372_RS12070 (2534709) 2534709..2536160 + 1452 WP_000854660.1 tagaturonate reductase -
HW372_RS12075 (2536357) 2536357..2537271 + 915 WP_001443133.1 bestrophin family protein -
HW372_RS12080 (2537275) 2537275..2538033 - 759 WP_001286540.1 trans-aconitate 2-methyltransferase -
HW372_RS12085 (2538341) 2538341..2538607 + 267 WP_001304368.1 type II toxin-antitoxin system antitoxin HipB Antitoxin
HW372_RS12090 (2538607) 2538607..2539929 + 1323 WP_001125431.1 type II toxin-antitoxin system serine/threonine protein kinase toxin HipA Toxin
HW372_RS12095 (2540239) 2540239..2540646 - 408 Protein_2371 acetylxylan esterase -
HW372_RS12100 (2540649) 2540649..2541230 - 582 Protein_2372 LysR family transcriptional regulator -
HW372_RS12105 (2541379) 2541379..2542161 + 783 WP_001221834.1 SDR family oxidoreductase -
HW372_RS12110 (2542405) 2542405..2542512 - 108 Protein_2374 hypothetical protein -
HW372_RS12115 (2542937) 2542937..2543500 + 564 WP_001327671.1 type 1 fimbrial major subunit FimA -
HW372_RS12120 (2543862) 2543862..2544572 + 711 WP_001195181.1 fimbria/pilus periplasmic chaperone -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(4-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(14-69)


Sequences

Toxin        


Download         Length: 441 a.a.        Molecular weight: 49310.02 Da        Isoelectric Point: 8.6972

>T166188 WP_001125431.1 NZ_CP058217:2538607-2539929 [Escherichia coli Nissle 1917]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLIPEDETVTCPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGRVRALAVKRFDRRW
NTERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIAQIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLKFPEVQMHEILSDFARMIP
AALDNVKNSLPTDFPENVVTAVETNVLRLHGRLSREYGIK

Download         Length: 1323 bp

>T166188 NZ_CP074076:c619849-619715 [Xanthomonas oryzae]
ATGAAGCGTGCAATTGTGCTGCTCGTGCTGTCCATGTTGTCGGTCGGTCTGCTGTCGGGCTGCAACACCGTCGCAGGTGC
CGGCAAGGACGTGAAGGGCGCCGGCGAGAAGGTGGAAGACGCAGCGCGTAAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9957.31 Da        Isoelectric Point: 4.9632

>AT166188 WP_001304368.1 NZ_CP058217:2538341-2538607 [Escherichia coli Nissle 1917]
MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTSLTTFFKILQSLELSMTLCDAKNASPEA
AEQQDLEW

Download         Length: 267 bp

>AT166188 NZ_CP074076:c620065-619913 [Xanthomonas oryzae]
ATGAAGCGACTGCTGACACTGATGGTGCTGGGCCTGTTTTCGGCCGGCGTGATGACGGGCTGCAACACCGTGGCTGGCGC
TGGCAAGGACTTGCAGGGCGCGGGTGGGAAGGTCGAGAAAACGGCCGAAAAATGCAGCGATGGTAAGTGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1L1KDK2

Antitoxin

Source ID Structure
AlphaFold DB V0VAH2

References