166172

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) spoIISABC/SpoIISA-SpoIISB
Location 3320331..3321248 Replicon chromosome
Accession NZ_CP058216
Organism Bacillus velezensis strain LF01

Toxin (Protein)


Gene name spoIISA Uniprot ID -
Locus tag HWH77_RS16980 Protein ID WP_025851786.1
Coordinates 3320502..3321248 (-) Length 249 a.a.

Antitoxin (Protein)


Gene name spoIISB Uniprot ID I2HQ14
Locus tag HWH77_RS16975 Protein ID WP_003154807.1
Coordinates 3320331..3320501 (-) Length 57 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HWH77_RS16940 3315562..3317184 + 1623 WP_069473361.1 pyocin knob domain-containing protein -
HWH77_RS16945 3317197..3317568 + 372 WP_014304856.1 hypothetical protein -
HWH77_RS16950 3317574..3317771 + 198 WP_003154819.1 XkdX family protein -
HWH77_RS16955 3317828..3318589 + 762 WP_033575307.1 hypothetical protein -
HWH77_RS16960 3318641..3318904 + 264 WP_003154815.1 hemolysin XhlA family protein -
HWH77_RS16965 3318918..3319181 + 264 WP_003154813.1 phage holin -
HWH77_RS16970 3319195..3320073 + 879 WP_024085195.1 N-acetylmuramoyl-L-alanine amidase -
HWH77_RS16975 3320331..3320501 - 171 WP_003154807.1 type II toxin-antitoxin system SpoIISB family antitoxin Antitoxin
HWH77_RS16980 3320502..3321248 - 747 WP_025851786.1 type II toxin-antitoxin system SpoIISA family toxin Toxin
HWH77_RS16985 3321353..3322351 - 999 WP_003154805.1 inorganic phosphate transporter -
HWH77_RS16990 3322364..3322981 - 618 WP_003154804.1 DUF47 domain-containing protein -
HWH77_RS16995 3323267..3324583 - 1317 WP_003154801.1 amino acid permease -
HWH77_RS17000 3324906..3325856 + 951 WP_015388352.1 ring-cleaving dioxygenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin SpoIISA, type II toxin-antitoxin system

(3-239)

Antitoxin

Antitoxin SpoIISB

(1-56)


Sequences

Toxin        


Download         Length: 249 a.a.        Molecular weight: 29048.53 Da        Isoelectric Point: 4.7003

>T166172 WP_025851786.1 NZ_CP058216:c3321248-3320502 [Bacillus velezensis]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVVGAMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYGEKMNVTASL
CEYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQDRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Download         Length: 747 bp

>T166172 NZ_CP074052:2902507-2902614 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGTGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6676.74 Da        Isoelectric Point: 10.9273

>AT166172 WP_003154807.1 NZ_CP058216:c3320501-3320331 [Bacillus velezensis]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Download         Length: 171 bp

>AT166172 NZ_CP074052:c2902457-2902400 [Escherichia coli]
TCTGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTGTACCTCTCAACGTGCGGGGG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB I2HQ14

References