Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 2563063..2563700 Replicon chromosome
Accession NZ_CP058216
Organism Bacillus velezensis strain LF01

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag HWH77_RS12980 Protein ID WP_003156187.1
Coordinates 2563350..2563700 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag HWH77_RS12975 Protein ID WP_003156188.1
Coordinates 2563063..2563344 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HWH77_RS12955 2559428..2560027 - 600 WP_003156193.1 rhomboid family intramembrane serine protease -
HWH77_RS12960 2560120..2560485 + 366 WP_003156192.1 holo-ACP synthase -
HWH77_RS12965 2560650..2561657 + 1008 WP_003156191.1 outer membrane lipoprotein carrier protein LolA -
HWH77_RS12970 2561774..2562943 + 1170 WP_003156189.1 alanine racemase -
HWH77_RS12975 2563063..2563344 + 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
HWH77_RS12980 2563350..2563700 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
HWH77_RS12985 2563818..2564639 + 822 WP_003156182.1 STAS domain-containing protein -
HWH77_RS12990 2564644..2565009 + 366 WP_003156180.1 RsbT antagonist protein RsbS -
HWH77_RS12995 2565012..2565413 + 402 WP_003156178.1 anti-sigma regulatory factor -
HWH77_RS13000 2565425..2566432 + 1008 WP_003156177.1 PP2C family protein-serine/threonine phosphatase -
HWH77_RS13005 2566496..2566825 + 330 WP_003156176.1 anti-sigma factor antagonist RsbV -
HWH77_RS13010 2566822..2567304 + 483 WP_161503396.1 anti-sigma B factor RsbW -
HWH77_RS13015 2567270..2568058 + 789 WP_003156171.1 RNA polymerase sigma factor SigB -
HWH77_RS13020 2568058..2568660 + 603 WP_003156170.1 SpoIIE family protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T166171 WP_003156187.1 NZ_CP058216:2563350-2563700 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T166171 NZ_CP074052:2901972-2902079 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCGGCACCGATCCTGGCGGGGATTATTACCGCAGTGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT166171 WP_003156188.1 NZ_CP058216:2563063-2563344 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT166171 NZ_CP074052:c2901919-2901858 [Escherichia coli]
TGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References