Detailed information of TA system TA166170

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): rlegTA/AbiEii-AbiEi
• Location: 1824924..1826397
• Replicon: chromosome
• Accession: NZ_CP058214
• Organism: Kaustia mangrovi strain R1DC25

Toxin (Protein)

• Gene name: rlegT
• Uniprot ID: -
• Locus tag: HW532_RS08510
• Protein ID: WP_213163966.1
• Coordinates: 1824924..1825787 (-)
• Length: 288 a.a.

Antitoxin (Protein)

• Gene name: rlegA
• Uniprot ID: -
• Locus tag: HW532_RS08515
• Protein ID: WP_213163967.1
• Coordinates: 1825780..1826397 (-)
• Length: 206 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HW532_RS22090	1820372..1821052	-	681	WP_246479742.1	DUF262 domain-containing protein	-
HW532_RS08495 (HW532_08475)	1821049..1822206	-	1158	WP_213163964.1	restriction endonuclease subunit S	-
HW532_RS08500 (HW532_08480)	1822200..1824608	-	2409	WP_213163965.1	type I restriction-modification system subunit M	-
HW532_RS08505 (HW532_08485)	1824589..1824795	-	207	WP_054522784.1	hypothetical protein	-
HW532_RS08510 (HW532_08490)	1824924..1825787	-	864	WP_213163966.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
HW532_RS08515 (HW532_08495)	1825780..1826397	-	618	WP_213163967.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	Antitoxin
HW532_RS08520 (HW532_08500)	1826653..1827489	-	837	WP_213163968.1	LysR family transcriptional regulator	-
HW532_RS08525 (HW532_08505)	1827588..1828568	+	981	WP_213163969.1	aldo/keto reductase	-
HW532_RS08530 (HW532_08510)	1828577..1829746	+	1170	WP_213163970.1	alpha/beta fold hydrolase	-
HW532_RS08535 (HW532_08515)	1829853..1831049	+	1197	WP_213163971.1	DUF932 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	1668529..1829896	161367

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (38-262)

Antitoxin

• Transcriptional regulator, AbiEi antitoxin (17-56)

Sequences

Toxin

Protein

• Length: 288 a.a.
• Molecular weight: 31991.69 Da
• Isoelectric Point: 4.8516

>T166170 WP_213163966.1 NZ_CP058214:c1825787-1824924 [Kaustia mangrovi]
MAKEMKNIGASVRARLLNLSKAKGQSFDLVLTRFALERLLFRLSQSPQADRFVLKGAMLMMSWFDDPHRGTRDLDLLGFG
DPSEAAMLATFRDILAQDAEDGVVFDPDTLRIDRIREELDYGGLRLRAIASVGGAQINLTIDIGFGDALEPGAEVVDYPA
MLDFPPPRLRAYARETVIAEKFQAMVALGRANSRMKDFYDIWVLSRSFSFDDDRLAQAIAATFARRKTPIPTELPDALTP
AFAADEQKQRQWRAFVEDLSVNPGELGDVVGELRVFLMPYASVARAI

Nucleotide

• Length: 864 bp

>T166170 NZ_CP074052:2179348-2179451 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 206 a.a.
• Molecular weight: 22185.64 Da
• Isoelectric Point: 7.9511

>AT166170 WP_213163967.1 NZ_CP058214:c1826397-1825780 [Kaustia mangrovi]
MPQTPSQREIAHDLLATHGILRLAELRDAGVTAATVSRMEKDGEVIRLARGLYQLPDAELDANHSLAEAAKRFPKGVICL
VSALAFHGLTDQLPKKVWMAIGRNDWTPKPSDMPIRVLRFSDDLLADSVETHVIEGVSVKVFGVAKTVADCFRHRGKIGL
PVALEGLQEALRQRKATPAEIARAADIGGVGTVIRPYLEALTANG

Nucleotide

• Length: 618 bp

>AT166170 NZ_CP074052:c2179489-2179218 [Escherichia coli]
AAAGTCAGCGAAGGGAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGCGGTGGTCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT