Detailed information of TA system TA166169

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 5268584..5269426
• Replicon: chromosome
• Accession: NZ_CP058212
• Organism: Klebsiella michiganensis strain RHB20-C02

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: HVX57_RS24725
• Protein ID: WP_040246536.1
• Coordinates: 5268584..5268961 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: HVX57_RS24730
• Protein ID: WP_077256055.1
• Coordinates: 5269013..5269426 (-)
• Length: 138 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HVX57_RS24690	5264010..5264333	+	324	WP_080528469.1	endoribonuclease SymE	-
HVX57_RS24695	5264492..5264926	+	435	WP_004098173.1	VOC family protein	-
HVX57_RS24700	5265092..5265835	+	744	WP_181251272.1	hypothetical protein	-
HVX57_RS24705	5266013..5266810	-	798	WP_047684029.1	helix-turn-helix transcriptional regulator	-
HVX57_RS24710	5266954..5267799	-	846	WP_181251273.1	DUF4942 domain-containing protein	-
HVX57_RS24715	5267881..5268066	-	186	WP_047684023.1	DUF957 domain-containing protein	-
HVX57_RS24720	5268096..5268587	-	492	WP_047684020.1	hypothetical protein	-
HVX57_RS24725	5268584..5268961	-	378	WP_040246536.1	TA system toxin CbtA family protein	Toxin
HVX57_RS24730	5269013..5269426	-	414	WP_077256055.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HVX57_RS24735	5269395..5269616	-	222	WP_040246532.1	DUF987 domain-containing protein	-
HVX57_RS24740	5269637..5270116	-	480	WP_040246529.1	DNA repair protein RadC	-
HVX57_RS24745	5270128..5270598	-	471	WP_134897158.1	antirestriction protein	-
HVX57_RS24750	5270838..5271029	-	192	WP_077256557.1	DUF905 family protein	-
HVX57_RS24755	5271085..5271306	-	222	WP_047684010.1	hypothetical protein	-
HVX57_RS24760	5271380..5271850	-	471	WP_094963531.1	hypothetical protein	-
HVX57_RS24765	5271902..5272213	-	312	WP_134897160.1	hypothetical protein	-
HVX57_RS24770	5272271..5272573	-	303	WP_134897161.1	hypothetical protein	-
HVX57_RS24775	5272606..5272884	-	279	WP_176451023.1	hypothetical protein	-
HVX57_RS24780	5273057..5273767	-	711	WP_047684001.1	WYL domain-containing protein	-
HVX57_RS24785	5274080..5274364	-	285	WP_134897228.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	5264492..5332759	68267

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (33-132)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14021.73 Da
• Isoelectric Point: 5.5540

>T166169 WP_040246536.1 NZ_CP058212:c5268961-5268584 [Klebsiella michiganensis]
MQTQPLSSTQEASPRPSQVDIWQQLLSHLLDRHYGLTLNDTPFGHDGVIQEHIDAGISLCDAVNVIVEKYDLVRTDRPGF
SAETQSPLISSIDILRARKATGLMTRNDYRTVTDITTGKYREVQP

Nucleotide

• Length: 378 bp

>T166169 NZ_CP074052:1995888-1996142 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 138 a.a.
• Molecular weight: 14964.07 Da
• Isoelectric Point: 6.8319

>AT166169 WP_077256055.1 NZ_CP058212:c5269426-5269013 [Klebsiella michiganensis]
MSSCAVSRSINVNLRKAKLSNEIPAVNHDIAEPWWGLKPTVTPCFGARLVQEGNQVHYLADRASITGQFSEADLRHLDQA
FPVLLKQLELMLVSGELNPHHQHGVTLYAKGLTCEADSLGSHGYIYIAIYPTPAATA

Nucleotide

• Length: 414 bp

>AT166169 NZ_CP074052:1995640-1995891 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TGCTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA