Detailed information of TA system TA166168

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 5212065..5212641
• Replicon: chromosome
• Accession: NZ_CP058212
• Organism: Klebsiella michiganensis strain RHB20-C02

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: -
• Locus tag: HVX57_RS24455
• Protein ID: WP_064375702.1
• Coordinates: 5212354..5212641 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: A0A0H3H591
• Locus tag: HVX57_RS24450
• Protein ID: WP_014227757.1
• Coordinates: 5212065..5212367 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HVX57_RS24435	5208906..5209241	+	336	WP_064375703.1	endoribonuclease SymE	-
HVX57_RS24440	5209693..5210604	+	912	WP_181251267.1	acetamidase/formamidase family protein	-
HVX57_RS24445	5210601..5211944	+	1344	WP_049101852.1	APC family permease	-
HVX57_RS24450	5212065..5212367	-	303	WP_014227757.1	BrnA antitoxin family protein	Antitoxin
HVX57_RS24455	5212354..5212641	-	288	WP_064375702.1	BrnT family toxin	Toxin
HVX57_RS24460	5212889..5213332	-	444	WP_064375701.1	FosA family fosfomycin resistance glutathione transferase	-
HVX57_RS24465	5213326..5214234	-	909	WP_064375700.1	LysR family transcriptional regulator	-
HVX57_RS24470	5214322..5215104	+	783	WP_181251268.1	NAD(P)H-dependent oxidoreductase	-
HVX57_RS24475	5215252..5215836	+	585	WP_004098242.1	TetR/AcrR family transcriptional regulator	-
HVX57_RS24480	5215982..5216782	+	801	WP_064375698.1	helix-turn-helix domain-containing protein	-
HVX57_RS24485	5216779..5217297	+	519	WP_004098240.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (38-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11210.69 Da
• Isoelectric Point: 7.4687

>T166168 WP_064375702.1 NZ_CP058212:c5212641-5212354 [Klebsiella michiganensis]
MPMEFEWDANKAISNLRKHGIRFEEAVLVFDDPRHLSRQDRYENGEYRWQTLGLVHGIIVIMVAHSVRFESGTEVIRIIS
ARKADSKERNRYEHG

Nucleotide

• Length: 288 bp

>T166168 NZ_CP074052:1995640-1995891 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TGCTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11607.18 Da
• Isoelectric Point: 9.4429

>AT166168 WP_014227757.1 NZ_CP058212:c5212367-5212065 [Klebsiella michiganensis]
MNMVKHKRDNPDTLTAKRDDELKALANKTDDEIDYSDIPATDDAQWSDAVRGKFYRPLKTQASVRIDADIMEWLKRPGKG
YQTRLNAILREAMIRDLNKK

Nucleotide

• Length: 303 bp

>AT166168 NZ_CP074052:1995888-1996142 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Structures

Antitoxin

• AlphaFold DB: A0A0H3H591