Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 2080607..2081197 Replicon chromosome
Accession NZ_CP058212
Organism Klebsiella michiganensis strain RHB20-C02

Toxin (Protein)


Gene name pemK Uniprot ID A0A7H5AAK0
Locus tag HVX57_RS09715 Protein ID WP_004852304.1
Coordinates 2080865..2081197 (+) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID J6I3K7
Locus tag HVX57_RS09710 Protein ID WP_004852307.1
Coordinates 2080607..2080864 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HVX57_RS09705 2080185..2080346 + 162 WP_134897734.1 helix-turn-helix domain-containing protein -
HVX57_RS09710 2080607..2080864 + 258 WP_004852307.1 antitoxin Antitoxin
HVX57_RS09715 2080865..2081197 + 333 WP_004852304.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
HVX57_RS09725 2081520..2082977 + 1458 WP_049079573.1 EmmdR/YeeO family multidrug/toxin efflux MATE transporter -
HVX57_RS09735 2083941..2085395 - 1455 WP_134897790.1 AMP nucleosidase -
HVX57_RS09740 2085528..2085785 - 258 WP_014229908.1 histidine kinase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-109)

Antitoxin

Antitoxin PemI-like

(4-84)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 11863.81 Da        Isoelectric Point: 10.5834

>T166157 WP_004852304.1 NZ_CP058212:2080865-2081197 [Klebsiella michiganensis]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPITSGGNFARTAGFTVSLEGAGIKTLGVIRCDQPRTI
DMTARNGKRLERIPDAVVNEVLARLDAILS

Download         Length: 333 bp

>T166157 NZ_CP074047:c4087336-4086986 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACACGCCCGGCAGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGAAATAA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9422.88 Da        Isoelectric Point: 4.4607

>AT166157 WP_004852307.1 NZ_CP058212:2080607-2080864 [Klebsiella michiganensis]
MYTTRLKKVGGSIMLAVPPAVLKTLELSTDSEVGMTINNGCLIIEPQKRPHYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Download         Length: 258 bp

>AT166157 NZ_CP074047:c4087581-4087330 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTTCAGGTATGGTCATTCCTAATGTTGTGATGAAAGAACTTAACTTACG
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCTACCCCTGCGGGTGAC
GAAATATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7H5AAK0

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3HDW8

References