Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 58340..58887 Replicon chromosome
Accession NZ_CP058212
Organism Klebsiella michiganensis strain RHB20-C02

Toxin (Protein)


Gene name ccdB Uniprot ID A0A0H3H5T3
Locus tag HVX57_RS00290 Protein ID WP_014227299.1
Coordinates 58340..58648 (-) Length 103 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID A0A0H3H0P7
Locus tag HVX57_RS00295 Protein ID WP_014227298.1
Coordinates 58651..58887 (-) Length 79 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HVX57_RS00265 54256..54567 - 312 WP_014227304.1 PTS sugar transporter subunit IIB -
HVX57_RS00270 54862..55803 + 942 WP_014227303.1 LacI family transcriptional regulator -
HVX57_RS00275 55827..56120 - 294 WP_014227302.1 hypothetical protein -
HVX57_RS00280 56330..57283 + 954 WP_032751828.1 Rpn family recombination-promoting nuclease/putative transposase -
HVX57_RS00285 57287..58189 - 903 WP_014227300.1 EamA family transporter -
HVX57_RS00290 58340..58648 - 309 WP_014227299.1 CcdB family protein Toxin
HVX57_RS00295 58651..58887 - 237 WP_014227298.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
HVX57_RS00300 58993..60426 - 1434 WP_032751833.1 PTS N-acetylmuramic acid transporter subunit IIBC -
HVX57_RS00305 60451..61353 - 903 WP_009654080.1 N-acetylmuramic acid 6-phosphate etherase -
HVX57_RS00310 61514..62680 - 1167 WP_181251347.1 multidrug effflux MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-101)

Antitoxin

Post-segregation antitoxin CcdA

(8-78)


Sequences

Toxin        


Download         Length: 103 a.a.        Molecular weight: 11725.57 Da        Isoelectric Point: 7.2729

>T166155 WP_014227299.1 NZ_CP058212:c58648-58340 [Klebsiella michiganensis]
MQYYVYKNTGRIAAYPYLLDVQSDIIGKRNTRVVIPLFPLKNYKGPRADRLTPLVTVEGEEYVVMTHELASIPHRVLGEE
VCNLNHQREVVKASMDFLFDGI

Download         Length: 309 bp

>T166155 NZ_CP074047:c3691512-3691114 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACAGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 79 a.a.        Molecular weight: 8783.92 Da        Isoelectric Point: 5.7928

>AT166155 WP_014227298.1 NZ_CP058212:c58887-58651 [Klebsiella michiganensis]
MPAAKRNKQNVSVTLDPGLVKQAREVGINLSLTLTNALKSEIHSLEIARWQKENAQAIDYLNNLTEENGLLSDEYRTF

Download         Length: 237 bp

>AT166155 NZ_CP074047:c3691808-3691515 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATTACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3H5T3

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3H0P7

References