Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 9009..9652 Replicon plasmid pRHB24-C12_3
Accession NZ_CP058211
Organism Escherichia marmotae strain RHB24-C12

Toxin (Protein)


Gene name vagD Uniprot ID B1LRW4
Locus tag HVY12_RS23620 Protein ID WP_001044768.1
Coordinates 9236..9652 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D2WFK3
Locus tag HVY12_RS23615 Protein ID WP_001261287.1
Coordinates 9009..9239 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HVY12_RS23585 4586..4999 + 414 WP_181238287.1 hydroxyisourate hydrolase -
HVY12_RS23590 5098..6090 + 993 WP_181238288.1 protein-methionine-sulfoxide reductase catalytic subunit MsrP -
HVY12_RS23595 6091..6726 + 636 WP_001559859.1 protein-methionine-sulfoxide reductase heme-binding subunit MsrQ -
HVY12_RS23600 7086..7892 - 807 WP_181238289.1 site-specific integrase -
HVY12_RS23605 7893..8198 - 306 WP_001159866.1 type II toxin-antitoxin system toxin CcdB -
HVY12_RS23610 8200..8418 - 219 WP_000813629.1 type II toxin-antitoxin system antitoxin CcdA -
HVY12_RS23615 9009..9239 + 231 WP_001261287.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
HVY12_RS23620 9236..9652 + 417 WP_001044768.1 type II toxin-antitoxin system VapC family toxin Toxin
HVY12_RS23625 9814..10975 - 1162 Protein_12 AAA family ATPase -
HVY12_RS23630 11036..11833 - 798 WP_000544799.1 IS21-like element IS21 family helper ATPase IstB -
HVY12_RS23635 11833..13005 - 1173 WP_047612361.1 IS21 family transposase -
HVY12_RS23640 13108..13383 - 276 Protein_15 AAA family ATPase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - faeJ / faeI / faeH / faeF / faeE / faeD / faeC 1..95282 95282


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15197.66 Da        Isoelectric Point: 7.7805

>T166150 WP_001044768.1 NZ_CP058211:9236-9652 [Escherichia marmotae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Download         Length: 417 bp

>T166150 NZ_CP074047:c2396609-2396433 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8815.90 Da        Isoelectric Point: 4.3775

>AT166150 WP_001261287.1 NZ_CP058211:9009-9239 [Escherichia marmotae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT166150 NZ_CP074047:c2396387-2395971 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A606Q844

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QFC4

References