Detailed information of TA system TA166149

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 7893..8418
• Replicon: plasmid pRHB24-C12_3
• Accession: NZ_CP058211
• Organism: Escherichia marmotae strain RHB24-C12

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: HVY12_RS23605
• Protein ID: WP_001159866.1
• Coordinates: 7893..8198 (-)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: A0A7W3G1F7
• Locus tag: HVY12_RS23610
• Protein ID: WP_000813629.1
• Coordinates: 8200..8418 (-)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HVY12_RS23580	3783..4454	-	672	WP_024188661.1	response regulator transcription factor HprR	-
HVY12_RS23585	4586..4999	+	414	WP_181238287.1	hydroxyisourate hydrolase	-
HVY12_RS23590	5098..6090	+	993	WP_181238288.1	protein-methionine-sulfoxide reductase catalytic subunit MsrP	-
HVY12_RS23595	6091..6726	+	636	WP_001559859.1	protein-methionine-sulfoxide reductase heme-binding subunit MsrQ	-
HVY12_RS23600	7086..7892	-	807	WP_181238289.1	site-specific integrase	-
HVY12_RS23605	7893..8198	-	306	WP_001159866.1	type II toxin-antitoxin system toxin CcdB	Toxin
HVY12_RS23610	8200..8418	-	219	WP_000813629.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
HVY12_RS23615	9009..9239	+	231	WP_001261287.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
HVY12_RS23620	9236..9652	+	417	WP_001044768.1	type II toxin-antitoxin system VapC family toxin	-
HVY12_RS23625	9814..10975	-	1162	Protein_12	AAA family ATPase	-
HVY12_RS23630	11036..11833	-	798	WP_000544799.1	IS21-like element IS21 family helper ATPase IstB	-
HVY12_RS23635	11833..13005	-	1173	WP_047612361.1	IS21 family transposase	-
HVY12_RS23640	13108..13383	-	276	Protein_15	AAA family ATPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	faeJ / faeI / faeH / faeF / faeE / faeD / faeC	1..95282	95282

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11673.44 Da
• Isoelectric Point: 6.2213

>T166149 WP_001159866.1 NZ_CP058211:c8198-7893 [Escherichia marmotae]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASAHLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T166149 NZ_CP074047:2310203-2311525 [Escherichia coli]
ATGCCTAAACTTGTCACTTGGATGAACAACCAGCGGGTAGGCGAGTTAACGAAGTTAGCCAACGGCGCGCACACCTTTAA
GTATGCACCGGAGTGGTTAGCAAGCCGTTATGCCAGACCGTTGTCACTTTCGCTGCCATTGCAGAGGGGGAATATCACCT
CTGATGCCGTATTTAACTTCTTCGATAACCTGTTACCCGATAGCCCGATTGTACGTGACCGGATCGTTAAACGTTATCAT
GCCAAATCCAGACAACCGTTTGATTTATTGTCAGAAATAGGGCGAGACAGCGTTGGTGCCGTGACGTTAATACCCGAAGA
CGAAACCGTAACGCATCCGATAATGGCATGGGAAAAGCTTACTGAAGCCAGACTTGAAGAAGTATTAACGGCTTATAAAG
CAGATATCCCGCTAGGCATGATTAGAGAAGAAAATGACTTTCGCATCTCGGTTGCTGGCGCACAGGAGAAGACAGCACTG
CTCAGAATAGGCAATGACTGGTGCATTCCGAAAGGAATAACGCCGACGACGCACATCATTAAATTACCGATTGGCGAAAT
CAGGCAGCCCAATGCGACGCTCGATCTCAGCCAAAGCGTTGATAATGAGTATTACTGTCTGCTGCTGGCGAAAGAACTTG
GGTTGAATGTTCCGGACGCAGAAATCATTAAAGCGGGAAATGTGCGCGCGTTAGCGGTCGAACGTTTTGACAGGCGTTGG
AATGCTGAGCGAACGGTTTTACTTCGCTTGCCACAGGAGGATATGTGTCAGACATTCGGTTTACCTTCATCGGTGAAATA
TGAATCAGATGGAGGCCCAGGCATCGCGCGGATCATGGCTTTTTTGATGGGGTCCAGCGAGGCGCTGAAAGATCGCTATG
ATTTTATGAAATTCCAGGTCTTCCAGTGGTTGATTGGCGCAACGGACGGTCATGCAAAAAACTTCTCCGTATTTATTCAG
GCTGGCGGCAGTTATCGACTCACGCCATTTTACGACATCATTTCAGCATTTCCGGTCCTTGGCGGTACGGGAATACACAT
CAGCGATCTCAAACTGGCAATGGGGCTTAACGCATCCAAAGGCAAAAAAACGGCAATCGATAAAATTTATCCGCGACATT
TTTTGGCGACAGCAAAGGTGCTGAGATTCCCGGAAGTGCAGATGCATGAAATCCTGAGTGACTTTGCCAGAATGATTCCA
GCAGCACTGGATAACGTGAAGACTTCATTACCGACAGATTTTCCGGAGAACGTGGTGACGGCAGTTGAAAGCAATGTGTT
GAGGTTGCATGGACGGTTAAGCCGAGAATACGGTAGTAAGTGA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8372.39 Da
• Isoelectric Point: 5.0807

>AT166149 WP_000813629.1 NZ_CP058211:c8418-8200 [Escherichia marmotae]
MKQRITVTVDSDSYKLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT166149 NZ_CP074047:2309937-2310203 [Escherichia coli]
ATGATGAGCTTTCAGAAGATCTATAGCCCAACGCAATTGGCGAATGCAATGAAACTGGTTCGCCAGCAAAATGGCTGGAC
GCAGAGCGAGCTGGCGAAAAAAATTGGTATTAAGCAGGCGACGATTTCCAATTTCGAAAACAACCCTGACAATACCACGC
TCACGACATTTTTTAAGATTTTACAGTCGCTTGAACTCTCAATGACGCTATGCGACGCGAAAAATGCCTCGCCAGAATCA
ACAGAACAGCAAAATCTGGAGTGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A7W3G1F7