Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 2522071..2522567 Replicon chromosome
Accession NZ_CP058209
Organism Escherichia marmotae strain RHB24-C12

Toxin (Protein)


Gene name ghoT Uniprot ID A0A7U9DCZ4
Locus tag HVY12_RS12360 Protein ID WP_001173345.1
Coordinates 2522071..2522244 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID A0A7U9DAJ1
Locus tag HVY12_RS12365 Protein ID WP_001517605.1
Coordinates 2522271..2522567 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HVY12_RS12350 2518741..2520198 + 1458 WP_001517606.1 dipeptide/tripeptide permease DtpC -
HVY12_RS12355 2520435..2521952 + 1518 WP_001295074.1 lysine--tRNA ligase -
HVY12_RS12360 2522071..2522244 - 174 WP_001173345.1 type V toxin-antitoxin system toxin GhoT Toxin
HVY12_RS12365 2522271..2522567 - 297 WP_001517605.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
HVY12_RS12370 2522795..2523067 - 273 WP_000405652.1 N-acetyltransferase -
HVY12_RS12375 2523079..2523309 - 231 WP_000371696.1 (4Fe-4S)-binding protein -
HVY12_RS12380 2523488..2525119 + 1632 WP_038355161.1 sensor histidine kinase -
HVY12_RS12385 2525116..2525835 + 720 WP_000611294.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(7-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T166142 WP_001173345.1 NZ_CP058209:c2522244-2522071 [Escherichia marmotae]
MALFSKILIFYVIGVNISFVIIWFLSHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T166142 NZ_CP074047:713010-713306 [Escherichia coli]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCCCTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11568.18 Da        Isoelectric Point: 4.0994

>AT166142 WP_001517605.1 NZ_CP058209:c2522567-2522271 [Escherichia marmotae]
MEGINRFKTYVVSFDYPSSYYSVFLRLRSLMYDMDFSSIVADEYGIPRQLNENAFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMIVDDYFRQFYK

Download         Length: 297 bp

>AT166142 NZ_CP074047:713308-713703 [Escherichia coli]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9DCZ4

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9DAJ1

References