Detailed information of TA system TA166141

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 2271039..2271615
• Replicon: chromosome
• Accession: NZ_CP058209
• Organism: Escherichia marmotae strain RHB24-C12

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: -
• Locus tag: HVY12_RS11100
• Protein ID: WP_181238172.1
• Coordinates: 2271328..2271615 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: A0A7U9DAW4
• Locus tag: HVY12_RS11095
• Protein ID: WP_000063150.1
• Coordinates: 2271039..2271341 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HVY12_RS11080	2267565..2269715	+	2151	WP_016249551.1	pyruvate/proton symporter BtsT	-
HVY12_RS11085	2269835..2270038	+	204	WP_000467859.1	YbdD/YjiX family protein	-
HVY12_RS11090	2270049..2271005	+	957	WP_001297640.1	GTPase	-
HVY12_RS11095	2271039..2271341	-	303	WP_000063150.1	BrnA antitoxin family protein	Antitoxin
HVY12_RS11100	2271328..2271615	-	288	WP_181238172.1	BrnT family toxin	Toxin
HVY12_RS11105	2271812..2272978	+	1167	WP_016249549.1	restriction endonuclease	-
HVY12_RS11110	2273042..2274661	+	1620	WP_001029743.1	type I restriction-modification system subunit M	-
HVY12_RS11115	2274651..2276012	+	1362	WP_181238173.1	restriction endonuclease subunit S	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (37-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11124.67 Da
• Isoelectric Point: 8.6424

>T166141 WP_181238172.1 NZ_CP058209:c2271615-2271328 [Escherichia marmotae]
MPMEFEWDANKVKSNRVKHGIRFEDAVLLFDDPQHLSQQDRVGNGEYRWQTIGLVHGIVVILVAHIIRFESGNEIIRIIS
ARKADRKERSRYEHG

Nucleotide

• Length: 288 bp

>T166141 NZ_CP074047:644798-645112 [Escherichia coli]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11532.03 Da
• Isoelectric Point: 9.4646

>AT166141 WP_000063150.1 NZ_CP058209:c2271341-2271039 [Escherichia marmotae]
MSMVKHKRGTPSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Nucleotide

• Length: 303 bp

>AT166141 NZ_CP074047:645109-645525 [Escherichia coli]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Structures

Antitoxin

• AlphaFold DB: A0A7U9DAW4