Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1733519..1734137 Replicon chromosome
Accession NZ_CP058209
Organism Escherichia marmotae strain RHB24-C12

Toxin (Protein)


Gene name Hha Uniprot ID V1H8E6
Locus tag HVY12_RS08625 Protein ID WP_001280991.1
Coordinates 1733919..1734137 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A0A7U9DNY0
Locus tag HVY12_RS08620 Protein ID WP_000344801.1
Coordinates 1733519..1733914 (+) Length 132 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HVY12_RS08610 1728597..1729790 + 1194 WP_001515899.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
HVY12_RS08615 1729813..1732962 + 3150 WP_001132495.1 multidrug efflux RND transporter permease subunit -
HVY12_RS08620 1733519..1733914 + 396 WP_000344801.1 Hha toxicity modulator TomB Antitoxin
HVY12_RS08625 1733919..1734137 + 219 WP_001280991.1 hemolysin expression modulator Hha Toxin
HVY12_RS08630 1734309..1734860 + 552 WP_000102537.1 maltose O-acetyltransferase -
HVY12_RS08635 1734975..1735445 + 471 WP_001515897.1 YlaC family protein -
HVY12_RS08640 1735609..1737159 + 1551 WP_001515896.1 EAL domain-containing protein -
HVY12_RS08645 1737196..1737549 - 354 WP_000878149.1 DUF1428 domain-containing protein -
HVY12_RS08655 1737929..1738240 + 312 WP_181238101.1 MGMT family protein -
HVY12_RS08660 1738271..1738843 - 573 WP_181238102.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8613.97 Da        Isoelectric Point: 8.9008

>T166138 WP_001280991.1 NZ_CP058209:1733919-1734137 [Escherichia marmotae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T166138 NZ_CP074043:45335-45856 [Escherichia coli]
GTGAGCAATACGACGATCGAGATTTTCTCTGGAGAGAAAGATTATGATCTAAACGGTTTTGATTGCGGCGAAGAGTCACT
AAACGCATTTTTGACCAACCACTTAAAAAGACAGCATGAAGGAAAAATTCTTCGCGCTTATGTGCTTTGCACTAAAGAAG
AAATACCAAAGGTATTAGGTTATTACACTTTGTCAGGCAGCTGCTTTGAGAAAGAAAACTTACCTTCAAAGAGTCAGCAA
AAGAAAGTGCCTTATCGGCATGTCCCAAGTATCACTTTAGGCAGACTGGCTTTGGACAAATCTCTTCAAGGTCAGGGGCT
TGGCTCAATGCTTGTAACTCACGCAATGCGTGTTGTGTACAATGCATCTCTTGCGGTAGGCATTCATGGGCTTTTCGTTG
AGGCGTTGAACGATAAAGCCAGGGCGTTTTATAAAAGTTTGGGCTTTATCCAGCTGGTTGGTAACAACGATCGTTCTTTG
TTCTATCCAACAAAATCTATCGAAAAATTGTTCGAAGAATAA

Antitoxin


Download         Length: 132 a.a.        Molecular weight: 15393.22 Da        Isoelectric Point: 4.9228

>AT166138 WP_000344801.1 NZ_CP058209:1733519-1733914 [Escherichia marmotae]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSCSNYYNHG

Download         Length: 396 bp

>AT166138 NZ_CP074043:45058-45327 [Escherichia coli]
ATGTCCGCACTGAAAAAGCAGCGCATCGATTTGAGATTAACCGATGACGATAAAAGCATCATCGAGGAAGCTGCCGCAAT
GTCTAACCAGAGCATTACCCAGTTCATGGTTAGCAGTGCATCCGAACGTGCCGTGAAGGTGATAGAGCAACACCGTAGAC
TGGTTCTAAGTGAAGAGTCCTGGAATCTGGTTATGGATGCTATAAGCAATCCTCCGGCACCGAACGACAAGCTGAAACGA
GCTGCCAATCGTCTGAAAAGCATGGAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4ICG

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9DNY0

References