Detailed information of TA system TA166124

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 479806..480444
• Replicon: chromosome
• Accession: NZ_CP058209
• Organism: Escherichia marmotae strain RHB24-C12

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A7U9DHD1
• Locus tag: HVY12_RS02465
• Protein ID: WP_000813795.1
• Coordinates: 479806..479982 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: HVY12_RS02470
• Protein ID: WP_077627724.1
• Coordinates: 480028..480444 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HVY12_RS02445	475426..476637	-	1212	WP_071990339.1	benzoate/H(+) symporter BenE family transporter	-
HVY12_RS02450	476690..477226	+	537	WP_000429073.1	XRE family transcriptional regulator	-
HVY12_RS02455	477299..479260	+	1962	WP_105224594.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
HVY12_RS02460	479354..479584	-	231	WP_000494240.1	DUF2554 family protein	-
HVY12_RS02465	479806..479982	+	177	WP_000813795.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
HVY12_RS02470	480028..480444	+	417	WP_077627724.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
HVY12_RS02475	480523..481932	+	1410	WP_061091001.1	PLP-dependent aminotransferase family protein	-
HVY12_RS02480	482171..483316	+	1146	WP_000047463.1	ABC transporter substrate-binding protein	-
HVY12_RS02485	483334..484347	+	1014	WP_016248715.1	ABC transporter ATP-binding protein	-
HVY12_RS02490	484348..485289	+	942	WP_181237914.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6747.80 Da
• Isoelectric Point: 11.5666

>T166124 WP_000813795.1 NZ_CP058209:479806-479982 [Escherichia marmotae]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPSEEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T166124 NZ_CP074042:1320571-1320969 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTGATTGAAGGTTTCGTATCTCGTTTGGAAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACT
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGATTGGAGCTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15250.62 Da
• Isoelectric Point: 4.5908

>AT166124 WP_077627724.1 NZ_CP058209:480028-480444 [Escherichia marmotae]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNNNDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT166124 NZ_CP074042:1320344-1320571 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAGGATGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTCTGA

Structures

Toxin

• AlphaFold DB: A0A7U9DHD1