Detailed information of TA system TA166119

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 3735220..3735998
• Replicon: chromosome
• Accession: NZ_CP058207
• Organism: Escherichia marmotae strain RHB42-C09

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: -
• Locus tag: HV018_RS17710
• Protein ID: WP_001517064.1
• Coordinates: 3735220..3735708 (-)
• Length: 163 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: A0A7H9KCB5
• Locus tag: HV018_RS17715
• Protein ID: WP_001110459.1
• Coordinates: 3735705..3735998 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV018_RS17695	3731089..3733224	-	2136	WP_181236964.1	ornithine decarboxylase	-
HV018_RS17700	3733623..3734330	+	708	WP_000234506.1	DUF554 domain-containing protein	-
HV018_RS17710	3735220..3735708	-	489	WP_001517064.1	GNAT family N-acetyltransferase	Toxin
HV018_RS17715	3735705..3735998	-	294	WP_001110459.1	DUF1778 domain-containing protein	Antitoxin
HV018_RS17720	3736533..3737030	-	498	WP_181237454.1	type VI secretion system contractile sheath small subunit	-
HV018_RS17725	3737623..3738096	+	474	WP_000143249.1	hypothetical protein	-
HV018_RS17730	3738093..3738428	+	336	WP_000789899.1	hypothetical protein	-
HV018_RS17735	3739253..3740236	+	984	WP_001325875.1	KpsF/GutQ family sugar-phosphate isomerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (87-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-88)

Sequences

Toxin

Protein

• Length: 163 a.a.
• Molecular weight: 17521.41 Da
• Isoelectric Point: 7.7535

>T166119 WP_001517064.1 NZ_CP058207:c3735708-3735220 [Escherichia marmotae]
MISAPEPLHAGHILTPFCCGVDSMDNWLKQRAMKNQVTGASRTFVCCGSDSKVLAYYSLASSAVMTNTAPGRFRRNMPDP
IPVVVLGRLAVDQSIHGQGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLLVTLGDLV
HA

Nucleotide

• Length: 489 bp

>T166119 NZ_CP074042:301961-302236 [Escherichia coli]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10949.56 Da
• Isoelectric Point: 9.9171

>AT166119 WP_001110459.1 NZ_CP058207:c3735998-3735705 [Escherichia marmotae]
MPAANSMAMKRETLNLRIRPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPEAYQTFLTRLDQAPAPN
SALRKTMQTPAPWEQEK

Nucleotide

• Length: 294 bp

>AT166119 NZ_CP074042:302233-302592 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCAGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCTAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Structures

Antitoxin

• AlphaFold DB: A0A7H9KCB5