Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 393717..394213 Replicon chromosome
Accession NZ_CP058207
Organism Escherichia marmotae strain RHB42-C09

Toxin (Protein)


Gene name ghoT Uniprot ID A0A7U9DCZ4
Locus tag HV018_RS01775 Protein ID WP_001173345.1
Coordinates 394040..394213 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID A0A7U9DAJ1
Locus tag HV018_RS01770 Protein ID WP_001517605.1
Coordinates 393717..394013 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV018_RS01750 390449..391168 - 720 WP_000611294.1 two-component system response regulator DcuR -
HV018_RS01755 391165..392796 - 1632 WP_038355161.1 sensor histidine kinase -
HV018_RS01760 392975..393205 + 231 WP_000371696.1 (4Fe-4S)-binding protein -
HV018_RS01765 393217..393489 + 273 WP_000405652.1 N-acetyltransferase -
HV018_RS01770 393717..394013 + 297 WP_001517605.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
HV018_RS01775 394040..394213 + 174 WP_001173345.1 type V toxin-antitoxin system toxin GhoT Toxin
HV018_RS01780 394332..395849 - 1518 WP_001295074.1 lysine--tRNA ligase -
HV018_RS01785 396086..397543 - 1458 WP_001517606.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 393217..403065 9848


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(7-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T166101 WP_001173345.1 NZ_CP058207:394040-394213 [Escherichia marmotae]
MALFSKILIFYVIGVNISFVIIWFLSHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T166101 NZ_CP074033:2507167-2507424 [Escherichia coli]
ATGAATTTATCCCGTCAGGAACAACATACCTTACACGTTCTCGCTAAAGGTAGACGTATTGCGCACGTCCGCGATTCTTC
AGGCCGCGTCACTTCCGTTGAATGCTACAGCCGCGAAGGGCTGTTGCTGACCGACTGCACGCTCGCCGTCTTCAAAAAAC
TCAAAACCAAAAAACTTATCAAGTCCGTCAATGGCCAGCCCTATCGAATCAACACCACCGAGCTGAATAAAGTTCGCGCT
CAGCTCGATAATCGCTAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11568.18 Da        Isoelectric Point: 4.0994

>AT166101 WP_001517605.1 NZ_CP058207:393717-394013 [Escherichia marmotae]
MEGINRFKTYVVSFDYPSSYYSVFLRLRSLMYDMDFSSIVADEYGIPRQLNENAFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMIVDDYFRQFYK

Download         Length: 297 bp

>AT166101 NZ_CP074033:2507436-2507981 [Escherichia coli]
ATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCATT
TGGCTTCAGCAAAGAAGCCGATTTAGTGGCCTCTTTACTTGAGGATGAAAGCGCGCGCCCCGCGTTATCGCTGCTAGCCC
GGTATGAAGGCAAAGCCGTCGGGCATATTTTATTTACCCGGGCAACCTTCAAAGGAGAAATGGATTCACCGTTGATGCAC
ATCCTCGCTCCCCTGGCGGTCATACCGGAATACCAAGGGATGGGCGTGGGAGGACGGTTGATACGCACTGGCATCGAGCA
CCTGAGGTTGATGGGCTGCCAGACGGTTTTTGTTCTCGGCCACGCCACCTATTACCCCCGACATGGGTTCGAACCCTGTG
CCGGAGACAAAGGCTATCCGGCACCTTATCCCATCCCGGAGGAGCACAAAGCCTGCTGGATGATGCAATCACTCACCGCT
CAGCCCATGACCCTGACCGGCCACATCCGGTGCGCAGACCCTGATGAAACCGGAGCACTGACGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9DCZ4

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9DAJ1

References