Detailed information of TA system TA166099

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 78558..79083
• Replicon: plasmid pRHBSTW-00002_3
• Accession: NZ_CP058198
• Organism: Enterobacter roggenkampii strain RHBSTW-00002

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: A0A7H9K2T5
• Locus tag: HV027_RS23220
• Protein ID: WP_045616569.1
• Coordinates: 78778..79083 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: A0A8H9ZZC6
• Locus tag: HV027_RS23215
• Protein ID: WP_016240352.1
• Coordinates: 78558..78776 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV027_RS23180	73922..74341	-	420	WP_011251286.1	helix-turn-helix domain-containing protein	-
HV027_RS23185	74338..74649	-	312	WP_011251285.1	type II toxin-antitoxin system HigB family toxin	-
HV027_RS23190	74854..75914	-	1061	Protein_72	IS3 family transposase	-
HV027_RS23195	75964..76380	-	417	WP_057072957.1	type II toxin-antitoxin system VapC family toxin	-
HV027_RS23200	76377..76607	-	231	WP_057072956.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
HV027_RS23210	78032..78388	+	357	Protein_76	hypothetical protein	-
HV027_RS23215	78558..78776	+	219	WP_016240352.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
HV027_RS23220	78778..79083	+	306	WP_045616569.1	type II toxin-antitoxin system toxin CcdB	Toxin
HV027_RS23225	79253..80269	+	1017	WP_063216424.1	hypothetical protein	-
HV027_RS23230	80467..81261	+	795	WP_016240356.1	site-specific integrase	-
HV027_RS23235	81472..82452	-	981	WP_000019450.1	IS5-like element ISKpn26 family transposase	-
HV027_RS23240	83118..83459	+	342	WP_152967296.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..108466	108466

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (5-92)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (4-84)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 13091.77 Da
• Isoelectric Point: 12.8043

>T166099 WP_045616569.1 NZ_CP058198:78778-79083 [Enterobacter roggenkampii]
KKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKK
KKKKKKKKKKKKKKKKKKKKKK

Nucleotide

• Length: 306 bp

>T166099 NZ_CP074033:2071394-2071672 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 9374.72 Da
• Isoelectric Point: 12.6573

>AT166099 WP_016240352.1 NZ_CP058198:78558-78776 [Enterobacter roggenkampii]
KKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKK

Nucleotide

• Length: 219 bp

>AT166099 NZ_CP074033:2071131-2071391 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Structures

Toxin

• AlphaFold DB: A0A7H9K2T5