Detailed information of TA system TA166098

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 75964..76607
• Replicon: plasmid pRHBSTW-00002_3
• Accession: NZ_CP058198
• Organism: Enterobacter roggenkampii strain RHBSTW-00002

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: HV027_RS23195
• Protein ID: WP_057072957.1
• Coordinates: 75964..76380 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: -
• Locus tag: HV027_RS23200
• Protein ID: WP_057072956.1
• Coordinates: 76377..76607 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV027_RS23160	72110..72580	-	471	WP_004883378.1	RES family NAD+ phosphorylase	-
HV027_RS23165	72577..73020	-	444	WP_032413443.1	DUF2384 domain-containing protein	-
HV027_RS23170	73121..73350	-	230	Protein_68	DUF4113 domain-containing protein	-
HV027_RS23175	73391..73708	+	318	Protein_69	transposase	-
HV027_RS23180	73922..74341	-	420	WP_011251286.1	helix-turn-helix domain-containing protein	-
HV027_RS23185	74338..74649	-	312	WP_011251285.1	type II toxin-antitoxin system HigB family toxin	-
HV027_RS23190	74854..75914	-	1061	Protein_72	IS3 family transposase	-
HV027_RS23195	75964..76380	-	417	WP_057072957.1	type II toxin-antitoxin system VapC family toxin	Toxin
HV027_RS23200	76377..76607	-	231	WP_057072956.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
HV027_RS23210	78032..78388	+	357	Protein_76	hypothetical protein	-
HV027_RS23215	78558..78776	+	219	WP_016240352.1	type II toxin-antitoxin system antitoxin CcdA	-
HV027_RS23220	78778..79083	+	306	WP_045616569.1	type II toxin-antitoxin system toxin CcdB	-
HV027_RS23225	79253..80269	+	1017	WP_063216424.1	hypothetical protein	-
HV027_RS23230	80467..81261	+	795	WP_016240356.1	site-specific integrase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..108466	108466

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (19-96)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 20475.56 Da
• Isoelectric Point: 5.5500

>T166098 WP_057072957.1 NZ_CP058198:c76380-75964 [Enterobacter roggenkampii]
FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF
FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF

Nucleotide

• Length: 417 bp

>T166098 NZ_CP074033:1888756-1889298 [Escherichia coli]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 11350.61 Da
• Isoelectric Point: 5.5500

>AT166098 WP_057072956.1 NZ_CP058198:c76607-76377 [Enterobacter roggenkampii]
FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF

Nucleotide

• Length: 231 bp

>AT166098 NZ_CP074033:1888461-1888772 [Escherichia coli]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACTAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA