Detailed information of TA system TA166094

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 1070..1620
• Replicon: plasmid pRHBSTW-00012_3
• Accession: NZ_CP058193
• Organism: Enterobacter hormaechei strain RHBSTW-00012

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: HV033_RS24440
• Protein ID: WP_181241457.1
• Coordinates: 1070..1378 (-)
• Length: 103 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: -
• Locus tag: HV033_RS24445
• Protein ID: WP_181241458.1
• Coordinates: 1381..1620 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV033_RS24430	217..702	+	486	WP_181241453.1	MbeB family mobilization protein	-
HV033_RS24435	763..999	+	237	WP_181241455.1	MbeD family mobilization/exclusion protein	-
HV033_RS24440	1070..1378	-	309	WP_181241457.1	CcdB family protein	Toxin
HV033_RS24445	1381..1620	-	240	WP_181241458.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
HV033_RS24450	1901..2833	+	933	WP_181241460.1	replication initiation protein	-
HV033_RS24455	2830..4986	+	2157	WP_181241462.1	MarR family transcriptional regulator	-
HV033_RS24460	5226..5552	+	327	WP_181241452.1	plasmid mobilization relaxosome protein MobC	-
HV033_RS24465	5542..6003	+	462	Protein_7	relaxase/mobilization nuclease domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..6013	6013

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-101)

Antitoxin

• Post-segregation antitoxin CcdA (9-79)

Sequences

Toxin

Protein

• Length: 103 a.a.
• Molecular weight: 11772.39 Da
• Isoelectric Point: 4.8307

>T166094 WP_181241457.1 NZ_CP058193:c1378-1070 [Enterobacter hormaechei]
MQFTVYRNNGNSRAYPYLLDVQSDIIGELKTRLVIPLYSLNEFSGRPARRLNPVLNVEDEDYLVMTHEMASVRLSQLGDE
VVSVREHREVIKNALDFLLDGF

Nucleotide

• Length: 309 bp

>T166094 NZ_CP074033:283234-283337 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9149.38 Da
• Isoelectric Point: 10.4450

>AT166094 WP_181241458.1 NZ_CP058193:c1620-1381 [Enterobacter hormaechei]
MTTSVMRHKKTVSVTLEPALLRQAREAGINLSATLATALRHEIRESESERWKRTNRKAIQELNRITDEHGLLSDDYRTF

Nucleotide

• Length: 240 bp

>AT166094 NZ_CP074033:c283375-283104 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTGAAATGTTAAAAACCGCCCG
TTCTGGTGAAATAACTGAGGCGGTTTTTTTAT