Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 847653..848456 Replicon chromosome
Accession NZ_CP058191
Organism Enterobacter hormaechei strain RHBSTW-00012

Toxin (Protein)


Gene name KacT Uniprot ID W8E895
Locus tag HV033_RS04090 Protein ID WP_016479963.1
Coordinates 847926..848456 (+) Length 177 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID W8E6Q5
Locus tag HV033_RS04085 Protein ID WP_022652312.1
Coordinates 847653..847922 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV033_RS04060 842955..843071 + 117 Protein_786 UTP--glucose-1-phosphate uridylyltransferase -
HV033_RS04065 843087..843347 - 261 Protein_787 IS1 family transposase -
HV033_RS04070 843409..844642 + 1234 Protein_788 IS3 family transposase -
HV033_RS04075 845348..846088 + 741 WP_104772627.1 tyrosine-type recombinase/integrase -
HV033_RS04080 846414..847319 - 906 WP_032072928.1 RepB family plasmid replication initiator protein -
HV033_RS04085 847653..847922 + 270 WP_022652312.1 DUF1778 domain-containing protein Antitoxin
HV033_RS04090 847926..848456 + 531 WP_016479963.1 GNAT family N-acetyltransferase Toxin
HV033_RS04095 848659..849645 - 987 WP_160391766.1 hypothetical protein -
HV033_RS04100 849642..849902 - 261 WP_063091378.1 hypothetical protein -
HV033_RS04105 850140..850640 + 501 WP_063091377.1 hypothetical protein -
HV033_RS04110 850653..851432 + 780 WP_104772726.1 hypothetical protein -
HV033_RS04115 851682..852379 + 698 Protein_797 IS1 family transposase -
HV033_RS04120 852454..852522 + 69 Protein_798 IS6 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - ugd 836186..871338 35152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(92-156)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 177 a.a.        Molecular weight: 20350.24 Da        Isoelectric Point: 6.4907

>T166077 WP_016479963.1 NZ_CP058191:847926-848456 [Enterobacter hormaechei]
MEGLRIEIFSEEVEYELSNFDCGEEYLNTFLTDHLKRQHNSKILRGYVLVTREDKPRVMGYYTLSGSCFEKILLPSKTQQ
KRVPYKNVPSVTLGRLAIDKSIHHQGYGETLVTHAMKVVYQASQAVGIHGMFVEALNDNAKKFYLRLGFIQLKEENCNSL
FYPTKSIEELFEVNDE

Download         Length: 531 bp

>T166077 NZ_CP074031:c2185788-2185681 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGGATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10269.70 Da        Isoelectric Point: 5.7252

>AT166077 WP_022652312.1 NZ_CP058191:847653-847922 [Enterobacter hormaechei]
MSALKKQRIDLRLNEDDKHMIEEAAAMTNQSISQFMVSTASERAAEVIDQHRRLLLNEESWNLVMDAIINPPAPNDRLKR
AANRLRELE

Download         Length: 270 bp

>AT166077 NZ_CP074031:2185835-2185901 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6C0L7Q2

Antitoxin

Source ID Structure
AlphaFold DB A0A2Z3XDS5

References