Detailed information of TA system TA166074

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 59497..60140
• Replicon: plasmid pRHBSTW-00016_2
• Accession: NZ_CP058188
• Organism: Enterobacter hormaechei strain RHBSTW-00016

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: HV036_RS23425
• Protein ID: WP_071687807.1
• Coordinates: 59724..60140 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: A0A6C0NE67
• Locus tag: HV036_RS23420
• Protein ID: WP_008322219.1
• Coordinates: 59497..59727 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV036_RS23395	54752..55075	+	324	WP_000143877.1	hypothetical protein	-
HV036_RS23400	55125..56498	+	1374	WP_040219225.1	RES family NAD+ phosphorylase	-
HV036_RS23405	56547..57764	-	1218	Protein_72	Y-family DNA polymerase	-
HV036_RS23410	57820..58788	-	969	WP_000654811.1	IS5 family transposase	-
HV036_RS23415	58864..59124	-	261	WP_040219223.1	type II toxin-antitoxin system ParD family antitoxin	-
HV036_RS23420	59497..59727	+	231	WP_008322219.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
HV036_RS23425	59724..60140	+	417	WP_071687807.1	type II toxin-antitoxin system VapC family toxin	Toxin
HV036_RS23430	60214..61776	+	1563	WP_008322224.1	AAA family ATPase	-
HV036_RS23435	61761..62783	+	1023	WP_008322227.1	helicase UvrD	-
HV036_RS23440	63036..63266	+	231	WP_001261276.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
HV036_RS23445	63263..63679	+	417	WP_008322233.1	type II toxin-antitoxin system VapC family toxin	-
HV036_RS23450	63873..64042	+	170	Protein_81	DUF596 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	sul1 / qacE / aadA2	mrkJ / mrkF / mrkD / mrkC / mrkB / mrkA	1..169857	169857
	-	flank	IS/Tn	-	-	57820..58788	968

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-50)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15156.50 Da
• Isoelectric Point: 7.1361

>T166074 WP_071687807.1 NZ_CP058188:59724-60140 [Enterobacter hormaechei]
VNKTYMLDTNICSFIMREQPEAVLKRLEQAVLRNQRIVVSAITYSEVRFGATGPKASPRHIELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Nucleotide

• Length: 417 bp

>T166074 NZ_CP074031:c2065480-2064986 [Escherichia coli]
ATGATTCAGGCCCCCGAGCCACTCATGGCAAGGCATCAATTCACCTCTTTTTGCTCGGGAGTAGAAACGATGGATAACTG
GCTAAAGCAGAGAGCCCTAAAAAATCAGCTTGCCGGCGCATCACGTACATTTGTTAGCTGTGATACGTATAGCAACGTTT
TGGCATATTATTCGCTTGCTTCCAGCGCCGTTGAAACGTATGTCGCGACAGGACGATTTCGCCGAAATATGCCAGAACCG
ATCCCTGTTGTAGTACTTGGTCGTCTGGCAATAGATAAATCATTACAGGGTCAGGGCATCGGTCGTGCGATGGTCAGAGA
TGCCGGTCTACGAGTACTACAGGCGGCTGAAGTCATTGGTATTCGCGGAATGTTGGTCCATGCGTTATCAGACCAGGCCC
GTGAGTTTTATCTGCGAGTGGGGTTTGAACCATCGCCTGTCGATTCTATGATACTGATGGCAACATTGGCTGATTTGCAG
GAATGTCTTAAATAG

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8736.84 Da
• Isoelectric Point: 4.4692

>AT166074 WP_008322219.1 NZ_CP058188:59497-59727 [Enterobacter hormaechei]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQLEKADPDFMAEREDVVTDEGRFNL

Nucleotide

• Length: 231 bp

>AT166074 NZ_CP074031:c2065770-2065477 [Escherichia coli]
ATGCCAGCAGCCAATAACATTCCAACCAGGCGCGAATCTTTAAATTTGCGCATAAAACCCGCAGAACGCGATCTTATTGA
CCGGGCCGCTAAAGCCCGAGGAAAAAATCGCACCGAATTTGTCCTTGAGGCCGCCAGAGCCGCAGCTGAAGAGGCGCTCA
TTGAGCAGAGAATTTTCAGGATCGATTCTGATGATTACCAGCTATTTTTGAATCGTCTGGATCAGCCCCCCGCGCCCAAT
GCTGCATTACGCAAAACCATGCAAACACCCGCGCCGTGGGAGCAAAAAAAATGA

Structures

Antitoxin

• AlphaFold DB: A0A6C0NE67