Detailed information of TA system TA166062

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 2314942..2315681
• Replicon: chromosome
• Accession: NZ_CP058187
• Organism: Enterobacter hormaechei strain RHBSTW-00016

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: HV036_RS11125
• Protein ID: WP_032624244.1
• Coordinates: 2314942..2315427 (-)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: A0A837FCR9
• Locus tag: HV036_RS11130
• Protein ID: WP_003857131.1
• Coordinates: 2315415..2315681 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV036_RS11095	2310503..2311087	-	585	WP_023306207.1	NUDIX hydrolase	-
HV036_RS11100	2311174..2311932	+	759	WP_023306208.1	DeoR/GlpR family DNA-binding transcription regulator	-
HV036_RS11105	2312037..2313329	+	1293	WP_023306209.1	hypothetical protein	-
HV036_RS11110	2313629..2313910	+	282	Protein_2162	DUF4225 domain-containing protein	-
HV036_RS11115	2313885..2314214	-	330	WP_023306210.1	hypothetical protein	-
HV036_RS11120	2314347..2314916	+	570	WP_023306211.1	hypothetical protein	-
HV036_RS11125	2314942..2315427	-	486	WP_032624244.1	GNAT family N-acetyltransferase	Toxin
HV036_RS11130	2315415..2315681	-	267	WP_003857131.1	DUF1778 domain-containing protein	Antitoxin
HV036_RS11135	2315745..2316674	-	930	WP_023306213.1	LysR family transcriptional regulator	-
HV036_RS11140	2316804..2318192	+	1389	WP_023306214.1	MFS transporter	-
HV036_RS11145	2318171..2318728	-	558	WP_023300131.1	OmpH family outer membrane protein	-
HV036_RS11150	2318849..2319985	-	1137	WP_032676232.1	type 1 fimbrial protein	-
HV036_RS11155	2320009..2320533	-	525	WP_087878309.1	fimbrial protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2298560..2315681	17121

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (58-151)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17531.27 Da
• Isoelectric Point: 10.1788

>T166062 WP_032624244.1 NZ_CP058187:c2315427-2314942 [Enterobacter hormaechei]
VGRVTAPEPLSSVHQLAEFVSGEAVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGNLRRNM
PDPIPVIILARLAVDVSIRGNGLGADLLQDAVLRCYRVAENIGVRAIMVHALTEKAKAFYIHHGFKASQTQERTLFLRLP
Q

Nucleotide

• Length: 486 bp

>T166062 NZ_CP074028:4325855-4326253 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10090.50 Da
• Isoelectric Point: 4.3837

>AT166062 WP_003857131.1 NZ_CP058187:c2315681-2315415 [Enterobacter hormaechei]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACQAAENVILDRRVFNFNDEQYAEFIDMLDAPVADDPAIEKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT166062 NZ_CP074028:4325628-4325855 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A837FCR9