Detailed information of TA system TA166059

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 20486..21222
• Replicon: plasmid pRHBSTW-00039_2
• Accession: NZ_CP058181
• Organism: Klebsiella grimontii strain RHBSTW-00039

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: A0A2J5Q928
• Locus tag: HV042_RS28805
• Protein ID: WP_004098919.1
• Coordinates: 20740..21222 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: A0A2J5Q9C0
• Locus tag: HV042_RS28800
• Protein ID: WP_004098921.1
• Coordinates: 20486..20752 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV042_RS28760	15743..16321	-	579	WP_009654302.1	hypothetical protein	-
HV042_RS28765	16611..16874	+	264	WP_009310051.1	hypothetical protein	-
HV042_RS28770	16871..17437	+	567	WP_009654312.1	hypothetical protein	-
HV042_RS28775	17468..17962	+	495	WP_013023776.1	hypothetical protein	-
HV042_RS28780	18006..18374	+	369	WP_063923539.1	hypothetical protein	-
HV042_RS28785	18405..18608	+	204	WP_004098931.1	hemolysin expression modulator Hha	-
HV042_RS28790	18657..18914	+	258	WP_004098928.1	hypothetical protein	-
HV042_RS28795	19143..20111	-	969	WP_163467572.1	IS5 family transposase	-
HV042_RS28800	20486..20752	+	267	WP_004098921.1	DUF1778 domain-containing protein	Antitoxin
HV042_RS28805	20740..21222	+	483	WP_004098919.1	GNAT family N-acetyltransferase	Toxin
HV042_RS28810	21381..22913	-	1533	WP_015065592.1	IS3-like element ISKpn38 family transposase	-
HV042_RS28815	23033..24379	+	1347	WP_077255298.1	ISNCY family transposase	-
HV042_RS28820	24438..25229	+	792	WP_003032641.1	tetratricopeptide repeat protein	-
HV042_RS28825	25270..26052	-	783	Protein_31	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	mrkJ / mrkF / mrkD / mrkC / mrkB / mrkA	1..133995	133995
	-	inside	IScluster/Tn	-	-	19143..26761	7618

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (66-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17335.06 Da
• Isoelectric Point: 10.0704

>T166059 WP_004098919.1 NZ_CP058181:20740-21222 [Klebsiella grimontii]
VGRITAPEPLSSAHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKKGTKKVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Nucleotide

• Length: 483 bp

>T166059 NZ_CP074028:c3635887-3635423 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTTTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAGACAGAAGAAAACCATTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10105.49 Da
• Isoelectric Point: 4.5141

>AT166059 WP_004098921.1 NZ_CP058181:20486-20752 [Klebsiella grimontii]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEIACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDDSAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT166059 NZ_CP074028:c3636222-3635887 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTCTTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: A0A2J5Q928

Antitoxin

• AlphaFold DB: A0A2J5Q9C0