Detailed information of TA system TA166058

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 5907039..5907649
• Replicon: chromosome
• Accession: NZ_CP058180
• Organism: Klebsiella grimontii strain RHBSTW-00039

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A9E1GAR7
• Locus tag: HV042_RS28270
• Protein ID: WP_049086919.1
• Coordinates: 5907039..5907413 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: HV042_RS28275
• Protein ID: WP_077253475.1
• Coordinates: 5907410..5907649 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV042_RS28255	5904194..5905096	+	903	WP_004122548.1	formate dehydrogenase subunit beta	-
HV042_RS28260	5905093..5905728	+	636	WP_004132860.1	formate dehydrogenase cytochrome b556 subunit	-
HV042_RS28265	5906072..5907001	+	930	WP_181334685.1	formate dehydrogenase accessory protein FdhE	-
HV042_RS28270	5907039..5907413	-	375	WP_049086919.1	type II toxin-antitoxin system VapC family toxin	Toxin
HV042_RS28275	5907410..5907649	-	240	WP_077253475.1	ribbon-helix-helix domain-containing protein	Antitoxin
HV042_RS28280	5907855..5908772	+	918	WP_024360706.1	alpha/beta hydrolase	-
HV042_RS28285	5908790..5909731	-	942	WP_181334686.1	fatty acid biosynthesis protein FabY	-
HV042_RS28290	5909776..5910213	-	438	WP_004132870.1	D-tyrosyl-tRNA(Tyr) deacylase	-
HV042_RS28295	5910210..5911070	-	861	WP_004127107.1	virulence factor BrkB family protein	-
HV042_RS28300	5911064..5911663	-	600	WP_004132872.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-110)

Antitoxin

• Ribbon-helix-helix protein, CopG (13-47)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 14111.45 Da
• Isoelectric Point: 8.4571

>T166058 WP_049086919.1 NZ_CP058180:c5907413-5907039 [Klebsiella grimontii]
MIKGPALFDTNILIDLFSGRVEAKQVLEAYPPQNAISLITWMEVMVGAKKYHQEHRTRIALSAFNIIDISQDIAERSVNL
RKEYGMKLPDAIILATAQIHRYELVTRNTKDFFGIPGVITPYHL

Nucleotide

• Length: 375 bp

>T166058 NZ_CP074028:3328787-3329314 [Escherichia coli]
ATGGATGATCTGACGATAGAGATTCTGACCGATGATGCAGATTATGATCTACAGCGATTCGACTGCGGCGAGGAAGCGTT
AAATCTCTTTCTGACGACACATCTCGTTCGTCAACATCGCAACAAAATTCTGCGAGCGTATATCCTTTGTCGCAACACTC
CAGAACGTCAGGTGCTGGGATATTACACATTATGCGGCAGTTGTTTTGAACGAGCCGCATTGCCCTCGAAATCGAAACAG
AAAAAAATTCCCTACAAAAATATTCCCAGCGTTACTCTTGGGCGTCTGGCAATTGATCGTTCATTACAGGGGCAGGGATG
GGGAGCAACACTGGTTGCTCATGCCATGAAAGTCGTCTGGTCAGCCTCTTTAGCGGTAGGTATTCACGGTCTTTTTGTCG
AGGCGCTGAATAAAAAAGCCCATACGTTTTATCAATCGCTGGGCTTTATCCCGTTGGTCGGAGAAAACGAAAATGCGTTA
TTTTTCCCAACCAAATCCATCGAACTGCTTTTCACACAGAGCGATTAA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9293.49 Da
• Isoelectric Point: 4.3142

>AT166058 WP_077253475.1 NZ_CP058180:c5907649-5907410 [Klebsiella grimontii]
MMTGTNMGRILLDLSDDVIKRLEDLKQQRNLPRAELLREAVEQYLERQADTTISNALGIWQGCEEDGVEFERKLREEWQ

Nucleotide

• Length: 240 bp

>AT166058 NZ_CP074028:3328514-3328780 [Escherichia coli]
ATGTCTGCTGTTAAAAAGCAGCGTATCGATCTGCGTTTAACTGACGATGACAAAAGTATGATTGAGGAAGCGGCAGCGAT
ATCTAATCAGTCCGTCAGCCAATTTATGTTGAACAGCGCTTCGCAGCGGGCTGCGGAAGTGATTGAACAGCATCGGCGGG
TGATCCTCAATGAGGAATCCTGGACGCGGGTGATGGATGCGCTGAGTAATCCACCGTCACCTGGTGAAAAGCTAAAACGT
GCGGCAAAACGTCTTCAGGGAATGTAA