Detailed information of TA system TA166044

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yfjZ/CbtA-CbeA
• Location: 46482..47185
• Replicon: plasmid pRHBSTW-00040_3
• Accession: NZ_CP058177
• Organism: Enterobacter hormaechei strain RHBSTW-00040

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: A0A853JWF2
• Locus tag: HV043_RS23940
• Protein ID: WP_032267594.1
• Coordinates: 46844..47185 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: HV043_RS23935
• Protein ID: WP_181241123.1
• Coordinates: 46482..46823 (+)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV043_RS23910	42493..43190	+	698	Protein_38	IS1 family transposase	-
HV043_RS23915	43198..43650	+	453	Protein_39	type IV secretion system DNA-binding domain-containing protein	-
HV043_RS23920	43695..44923	+	1229	WP_155024100.1	IS3 family transposase	-
HV043_RS23925	45686..45916	+	231	Protein_41	antirestriction protein	-
HV043_RS23930	45929..46471	+	543	WP_032267592.1	DNA repair protein RadC	-
HV043_RS23935	46482..46823	+	342	WP_181241123.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HV043_RS23940	46844..47185	+	342	WP_032267594.1	TA system toxin CbtA family protein	Toxin
HV043_RS23945	47436..47546	+	111	Protein_45	restriction endonuclease	-
HV043_RS23950	47610..48590	+	981	WP_000019450.1	IS5-like element ISKpn26 family transposase	-
HV043_RS23955	49057..49368	+	312	WP_155024128.1	type II toxin-antitoxin system HigB family toxin	-
HV043_RS23960	49365..49784	+	420	WP_155024127.1	helix-turn-helix domain-containing protein	-
HV043_RS23965	49788..49991	-	204	WP_045898927.1	hypothetical protein	-
HV043_RS23970	50271..51275	-	1005	WP_022542389.1	IS110-like element IS4321 family transposase	-
HV043_RS23975	51375..52079	+	705	Protein_51	IS6-like element IS26 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	ugd / wbtL	1..60669	60669
	-	inside	IScluster/Tn	-	-	38045..54643	16598

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-107)

Antitoxin

• Antitoxin CbeA (13-110)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12767.68 Da
• Isoelectric Point: 6.8699

>T166044 WP_032267594.1 NZ_CP058177:46844-47185 [Enterobacter hormaechei]
MKTLPATTPQAATLCLSPVAVWQMLLARLLEQHYGLTLNDTPFSDEKVIQEHIDAGISLADAVNFLVEKYGLVRIDRRGF
GWQEQSPYLRAVDILRARQATGLLKARHGEHWE

Nucleotide

• Length: 342 bp

>T166044 NZ_CP074028:301985-302088 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 12746.43 Da
• Isoelectric Point: 6.6213

>AT166044 WP_181241123.1 NZ_CP058177:46482-46823 [Enterobacter hormaechei]
MKSTDSENDSFLKWGLNRNVTPCFGARLVQEGNRLHYLADRASITGKFSEAECLKLDLAFPHFISQMESMLTTGEINPRH
AHCVTLYHNGFTCEADSLGSCGYVYIAVYLTYR

Nucleotide

• Length: 342 bp

>AT166044 NZ_CP074028:c302126-301855 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTGAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Structures

Toxin

• AlphaFold DB: A0A853JWF2