Detailed information of TA system TA166031

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 93647..94366
• Replicon: chromosome
• Accession: NZ_CP058175
• Organism: Enterobacter hormaechei strain RHBSTW-00040

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A144XIR2
• Locus tag: HV043_RS00475
• Protein ID: WP_023305176.1
• Coordinates: 93647..93967 (-)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: HV043_RS00480
• Protein ID: WP_032660963.1
• Coordinates: 94001..94366 (-)
• Length: 122 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV043_RS00440	89540..90328	+	789	WP_115168434.1	winged helix-turn-helix domain-containing protein	-
HV043_RS00445	90330..90809	+	480	WP_022646606.1	hypothetical protein	-
HV043_RS00450	91052..91933	+	882	WP_032670896.1	helix-turn-helix domain-containing protein	-
HV043_RS00455	91998..92297	-	300	WP_023299004.1	XRE family transcriptional regulator	-
HV043_RS00460	92421..92762	-	342	WP_023305175.1	SymE family type I addiction module toxin	-
HV043_RS00465	92890..93051	+	162	WP_065212817.1	DUF3950 domain-containing protein	-
HV043_RS00470	93164..93370	-	207	WP_071788007.1	hypothetical protein	-
HV043_RS00475	93647..93967	-	321	WP_023305176.1	TA system toxin CbtA family protein	Toxin
HV043_RS00480	94001..94366	-	366	WP_032660963.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HV043_RS00485	94398..94619	-	222	WP_023305178.1	DUF987 family protein	-
HV043_RS00490	94628..95098	-	471	WP_023305179.1	DNA repair protein RadC	-
HV043_RS00495	95168..95989	-	822	WP_023305180.1	DUF945 domain-containing protein	-
HV043_RS00500	96432..96623	-	192	WP_023305181.1	hypothetical protein	-
HV043_RS00505	96682..97521	-	840	WP_050582604.1	hypothetical protein	-
HV043_RS00510	97634..98068	-	435	WP_023305183.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	88091..114448	26357

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (5-104)

Antitoxin

• Antitoxin CbeA (18-116)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 12190.77 Da
• Isoelectric Point: 5.9559

>T166031 WP_023305176.1 NZ_CP058175:c93967-93647 [Enterobacter hormaechei]
MHISTVPATVTVSSRLSPVQVWQKLLTYILEHHYGLTINDTPFSNDTTIQEHIEAGVNLTDAVNFLVERFELVRIDQKGF
SWQDQEPWITSLDVHRAQFNLGLKRS

Nucleotide

• Length: 321 bp

>T166031 NZ_CP074024:2378840-2380162 [Escherichia coli]
ATGCCTAAACTTGTCACTTGGATGAACAACCAGCGGGTAGGCGAGTTAACGAAGTTAGCCAACGGCGCGCACACCTTTAA
GTATGCACCGGAGTGGTTAGCAAGCCGTTATGCCAGACCGTTGTCACTTTCGCTGCCATTGCAGAGGGGGAATATCACCT
CTGATGCCGTATTTAACTTCTTCGATAACCTGTTACCCGATAGCCCGATTGTACGTGACCGGATCGTTAAACGTTATCAT
GCCAAATCCAGACAACCGTTTGATTTATTGTCAGAAATAGGGCGAGACAGCGTTGGTGCCGTGACGTTAATACCCGAAGA
CGAAACCGTAACGCATCCGATAATGGCATGGGAAAAGCTTACTGAAGCCAGACTTGAAGAAGTATTAACGGCTTATAAAG
CAGATATCCCGCTAGGCATGATTAGAGAAGAAAATGACTTTCGCATCTCGGTTGCTGGCGCACAGGAGAAGACAGCACTG
CTCAGAATAGGCAATGACTGGTGCATTCCGAAAGGAATAACGCCGACGACGCACATCATTAAATTACCGATTGGCGAAAT
CAGGCAGCCCAATGCGACGCTCGATCTCAGCCAAAGCGTTGATAATGAGTATTACTGTCTGCTGCTGGCGAAAGAACTTG
GGTTGAATGTTCCGGACGCAGAAATCATTAAAGCGGGAAATGTGCGCGCGTTAGCGGTCGAACGTTTTGACAGGCGTTGG
AATGCTGAGCGAACGGTTTTACTTCGCTTGCCACAGGAGGATATGTGTCAGACATTCGGTTTACCTTCATCGGTGAAATA
TGAATCAGATGGAGGCCCAGGCATCGCGCGGATCATGGCTTTTTTGATGGGGTCCAGCGAGGCGCTGAAAGATCGCTATG
ATTTTATGAAATTCCAGGTCTTCCAGTGGTTGATTGGCGCAACGGACGGTCATGCAAAAAACTTCTCCGTATTTATTCAG
GCTGGCGGCAGTTATCGACTCACGCCATTTTACGACATCATTTCAGCATTTCCGGTCCTTGGCGGTACGGGAATACACAT
CAGCGATCTCAAACTGGCAATGGGGCTTAACGCATCCAAAGGCAAAAAAACGGCAATCGATAAAATTTATCCGCGACATT
TTTTGGCGACAGCAAAGGTGCTGAGATTCCCGGAAGTGCAGATGCATGAAATCCTGAGTGACTTTGCCAGAATGATTCCA
GCAGCACTGGATAACGTGAAGACTTCATTACCGACAGATTTTCCGGAGAACGTGGTGACGGCAGTTGAAAGCAATGTGTT
GAGGTTGCATGGACGGTTAAGCCGAGAATACGGTAGTAAGTGA

Antitoxin

Protein

• Length: 122 a.a.
• Molecular weight: 13456.20 Da
• Isoelectric Point: 6.8421

>AT166031 WP_032660963.1 NZ_CP058175:c94366-94001 [Enterobacter hormaechei]
MQSTTLSGTSAENASCLQWGLKRNITPCFGARLVQEGNRLYFLADRAGFNGSFTDDEALRLDQAFPLMMKQLERMLTAGE
LDPRSQHCVTRHHNGLTCEADTLGSHGYVYIAIYPQSALTR

Nucleotide

• Length: 366 bp

>AT166031 NZ_CP074024:2378574-2378840 [Escherichia coli]
ATGATGAGCTTTCAGAAGATCTATAGCCCAACGCAATTGGCGAATGCAATGAAACTGGTTCGCCAGCAAAATGGCTGGAC
GCAGAGCGAGCTGGCGAAAAAAATTGGTATTAAGCAGGCGACGATTTCCAATTTCGAAAACAACCCTGACAATACCACGC
TCACGACATTTTTTAAGATTTTACAGTCGCTTGAACTCTCAATGACGCTATGCGACGCGAAAAATGCCTCGCCAGAATCA
ACAGAACAGCAAAATCTGGAGTGGTAA

Structures

Toxin

• AlphaFold DB: A0A144XIR2