Detailed information of TA system TA166019

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 2519302..2520041
• Replicon: chromosome
• Accession: NZ_CP058172
• Organism: Enterobacter hormaechei strain RHBSTW-00056

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: HV047_RS12640
• Protein ID: WP_155025303.1
• Coordinates: 2519302..2519787 (-)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: A0A837FCR9
• Locus tag: HV047_RS12645
• Protein ID: WP_003857131.1
• Coordinates: 2519775..2520041 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV047_RS12620	2515212..2515970	-	759	WP_032624253.1	trans-aconitate 2-methyltransferase	-
HV047_RS12625	2516055..2516639	-	585	WP_181242344.1	GDP-mannose pyrophosphatase	-
HV047_RS12630	2516726..2517484	+	759	WP_155025301.1	DeoR/GlpR family DNA-binding transcription regulator	-
HV047_RS12635	2517589..2518881	+	1293	WP_155025302.1	glycoside hydrolase	-
HV047_RS12640	2519302..2519787	-	486	WP_155025303.1	GNAT family N-acetyltransferase	Toxin
HV047_RS12645	2519775..2520041	-	267	WP_003857131.1	DUF1778 domain-containing protein	Antitoxin
HV047_RS12650	2520105..2521034	-	930	WP_155025304.1	LysR family transcriptional regulator	-
HV047_RS12655	2521164..2522552	+	1389	WP_155025305.1	MFS transporter	-
HV047_RS12660	2522531..2523088	-	558	WP_045622668.1	OmpH family outer membrane protein	-
HV047_RS12665	2523209..2524345	-	1137	WP_155025306.1	type 1 fimbrial protein	-
HV047_RS12670	2524369..2524893	-	525	WP_155025390.1	fimbrial protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (58-151)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17557.35 Da
• Isoelectric Point: 10.1788

>T166019 WP_155025303.1 NZ_CP058172:c2519787-2519302 [Enterobacter hormaechei]
VGRVTAPEPLSIVHQLAEFVSGEAVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGNLRRNM
PDPIPVIILARLAVDVSIRGNGLGADLLQDAVLRCYRVAENIGVRAIMVHALTEKAKAFYIHHGFKASQTQERTLFLRLP
Q

Nucleotide

• Length: 486 bp

>T166019 NZ_CP074020:21332-21748 [Escherichia coli]
GTGAACAAGACTTATATGCTTGATACCTGTATCTGCTCGTTCATCATGCGTGAACAGCCAGAAGCCGTCCTGAAGCGTCT
GGAGCAGGCGGTGCTACGCGGCCACCGTATAGTGGTCTCGGCCATCACCTACTCTGAAATGCGCTTCGGGGCTACCGGCC
CAAAGGCCTCACCACGTCACGTCCAACTGGTCGATGAATTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCC
GCAGTAGACGCCACCACGAAGATTAAAGTGGCACTGCGGCTGGCCGGGACGCCGATCGGCCCGAACGACACGGCGATTGC
CGGGCACGCCATCGCCGCCGGGGCGATACTGGTAACGAATAATACAAGAGAATTTGAGCGAGTGCCTGACCTGGTGCTGG
AAGACTGGGTGAAGTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10090.50 Da
• Isoelectric Point: 4.3837

>AT166019 WP_003857131.1 NZ_CP058172:c2520041-2519775 [Enterobacter hormaechei]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACQAAENVILDRRVFNFNDEQYAEFIDMLDAPVADDPAIEKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT166019 NZ_CP074020:21105-21335 [Escherichia coli]
ATGAGAACCGTATCGATATTCAAAAATGGCAACAACCGCGCCATCCGTCTGCCTCGTGATCTGGATTTTGAGGGGGTGAG
CGAGCTCGAGATCGTCCGGGAAGGGGACAGCATCATCCTGCGCCCCGTCCGGCCAACCTGGGGATCATTCCTAGAGTACG
AAAAAGCCGATCCAGATTTTATGGCGGAGCGCGAGGATGTTGTCAGCGACGAAGGGCGATTTAACCTGTGA

Structures

Antitoxin

• AlphaFold DB: A0A837FCR9